Melatonin and IL-25 modulate apoptosis and angiogenesis mediators in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumour cells

G. B. Gelaleti, Thaiz Ferraz Borin, L. B. Maschio-Signorini, M. G. Moschetta, E. Hellmén, A. M. Viloria-Petit, D. A.P.C. Zuccari

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Melatonin has oncostatic actions and IL-25 is active in inflammatory processes that induce apoptosis in tumor cells. Aim: The aim of this study was to evaluate melatonin and IL-25 in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumor cells cultured as monolayers and tridimensional structures. Materials and Methods: The cells were treated with melatonin, IL-25 and IL-17B silencing gene and performed cell viability, gene and protein expression of caspase-3 and VEGFA (Vascular endothelial growth factor A) and an apoptosis membrane protein array. Results: Treatment with 1 mM of melatonin reduced cell viability of both tumor cell lines, all treatments alone and combined significantly increased caspase-3 cleaved and proteins involved in the apoptotic pathway and reduced pro-angiogenic VEGFA, confirming the effectiveness of these potential promising treatments. Conclusion: This is the first study evaluating the potential use of these strategies in CF-41 and CMT-U229 cell lines and together encourages subsequent in vitro and in vivo studies for further exploration of clinical applications.

Original languageEnglish (US)
Pages (from-to)1572-1584
Number of pages13
JournalVeterinary and Comparative Oncology
Volume15
Issue number4
DOIs
StatePublished - Dec 1 2017

Fingerprint

mammary neoplasms (animal)
melatonin
Melatonin
angiogenesis
Canidae
apoptosis
vascular endothelial growth factor A
Apoptosis
Breast Neoplasms
dogs
caspase-3
Caspase 3
Vascular Endothelial Growth Factor A
cell viability
Cell Survival
cell lines
Protein Array Analysis
Interleukin-17
Gene Silencing
gene silencing

Keywords

  • angiogenesis
  • apoptosis
  • canine
  • interleukin-25
  • mammary tumour cells
  • melatonin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Melatonin and IL-25 modulate apoptosis and angiogenesis mediators in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumour cells. / Gelaleti, G. B.; Borin, Thaiz Ferraz; Maschio-Signorini, L. B.; Moschetta, M. G.; Hellmén, E.; Viloria-Petit, A. M.; Zuccari, D. A.P.C.

In: Veterinary and Comparative Oncology, Vol. 15, No. 4, 01.12.2017, p. 1572-1584.

Research output: Contribution to journalArticle

Gelaleti, G. B. ; Borin, Thaiz Ferraz ; Maschio-Signorini, L. B. ; Moschetta, M. G. ; Hellmén, E. ; Viloria-Petit, A. M. ; Zuccari, D. A.P.C. / Melatonin and IL-25 modulate apoptosis and angiogenesis mediators in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumour cells. In: Veterinary and Comparative Oncology. 2017 ; Vol. 15, No. 4. pp. 1572-1584.
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