Abstract
Background: Melatonin has oncostatic actions and IL-25 is active in inflammatory processes that induce apoptosis in tumor cells. Aim: The aim of this study was to evaluate melatonin and IL-25 in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumor cells cultured as monolayers and tridimensional structures. Materials and Methods: The cells were treated with melatonin, IL-25 and IL-17B silencing gene and performed cell viability, gene and protein expression of caspase-3 and VEGFA (Vascular endothelial growth factor A) and an apoptosis membrane protein array. Results: Treatment with 1 mM of melatonin reduced cell viability of both tumor cell lines, all treatments alone and combined significantly increased caspase-3 cleaved and proteins involved in the apoptotic pathway and reduced pro-angiogenic VEGFA, confirming the effectiveness of these potential promising treatments. Conclusion: This is the first study evaluating the potential use of these strategies in CF-41 and CMT-U229 cell lines and together encourages subsequent in vitro and in vivo studies for further exploration of clinical applications.
Original language | English (US) |
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Pages (from-to) | 1572-1584 |
Number of pages | 13 |
Journal | Veterinary and Comparative Oncology |
Volume | 15 |
Issue number | 4 |
DOIs | |
State | Published - Dec 1 2017 |
Keywords
- angiogenesis
- apoptosis
- canine
- interleukin-25
- mammary tumour cells
- melatonin
ASJC Scopus subject areas
- veterinary(all)