Melatonin regulates angiogenic and inflammatory proteins in MDA-MB-231 cell line and in co-culture with cancer-associated fibroblasts

Larissa B. Maschio-Signorini, Gabriela B. Gelaleti, Marina G. Moschetta, Thaiz Ferraz Borin, Bruna V. Jardim-Perassi, Juliana R. Lopes, Jessica Z. Lacerda, Rosimeire A. Roela, Newton A. Bordin, Lizandra A. Corrêa, Jessica P. Cardoso, Debora Aparecida Pires De Campos Zuccari

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Cancer-associated fibroblast (CAFs) are the most abundant cells in the tumor microenvironment, able to secrete growth factors and act on tumor progression. Melatonin is associated with several mechanisms of action with oncostatics and oncoprotectors effects, and also participate in the reduction of synthesis of surrounding fibroblasts and endothelial cells in breast cancer. Objective: The objectives of this study were to determine the effectiveness of melatonin in cell viability and expression of proteins involved in angiogenesis and inflammation in triplenegative mammary tumor cell line (MDA-MB-231) and in co-culture with CAFs. Method: Cell viability was measured by MTT assay and the protein expression was evaluated by Membrane Antibody Array after melatonin treatment. Results: Melatonin treatment (1 mM) for 48 hours reduced the cell viability of MDA-MB-231, CAFs and co-culture (p < 0.05). The semi-quantitative protein analysis showed that when monoculture of tumor cells were compared with co-culture of CAFs, there was a regulation of angiogenic and inflammatory proteins (p < 0.05). Melatonin treatment also leads a differential expression of angiogenic and inflammatory proteins in both monoculture and co-culture of tumor cells and CAFs (p < 0.05). Conclusion: The influence of CAFs under the tumor microenvironment was confirmed, increasing the malignancy of the tumor. In addition, melatonin is effective in both monoculture and co-culture, regulating angiogenic and inflammatory proteins that contribute to tumor progression. This study show an overview of melatonin ability in regulating angiogenic and inflammatory proteins, and opens the way for exploration of each individual protein in further studies.

Original languageEnglish (US)
Pages (from-to)1474-1484
Number of pages11
JournalMini-Reviews in Medicinal Chemistry
Volume16
Issue number17
DOIs
StatePublished - Nov 1 2016
Externally publishedYes

Fingerprint

Angiogenic Proteins
Melatonin
Coculture Techniques
Cell Line
Neoplasms
Cell Survival
Tumor Microenvironment
Proteins
Breast Neoplasms
Cancer-Associated Fibroblasts
Tumor Cell Line
Intercellular Signaling Peptides and Proteins
Endothelial Cells
Fibroblasts
Inflammation
Membranes
Antibodies

Keywords

  • Angiogenesis
  • Breast cancer
  • Co-culture
  • Inflammation
  • Melatonin
  • Microenvironment

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Maschio-Signorini, L. B., Gelaleti, G. B., Moschetta, M. G., Borin, T. F., Jardim-Perassi, B. V., Lopes, J. R., ... De Campos Zuccari, D. A. P. (2016). Melatonin regulates angiogenic and inflammatory proteins in MDA-MB-231 cell line and in co-culture with cancer-associated fibroblasts. Mini-Reviews in Medicinal Chemistry, 16(17), 1474-1484. https://doi.org/10.2174/1871520616666160422105920

Melatonin regulates angiogenic and inflammatory proteins in MDA-MB-231 cell line and in co-culture with cancer-associated fibroblasts. / Maschio-Signorini, Larissa B.; Gelaleti, Gabriela B.; Moschetta, Marina G.; Borin, Thaiz Ferraz; Jardim-Perassi, Bruna V.; Lopes, Juliana R.; Lacerda, Jessica Z.; Roela, Rosimeire A.; Bordin, Newton A.; Corrêa, Lizandra A.; Cardoso, Jessica P.; De Campos Zuccari, Debora Aparecida Pires.

In: Mini-Reviews in Medicinal Chemistry, Vol. 16, No. 17, 01.11.2016, p. 1474-1484.

Research output: Contribution to journalArticle

Maschio-Signorini, LB, Gelaleti, GB, Moschetta, MG, Borin, TF, Jardim-Perassi, BV, Lopes, JR, Lacerda, JZ, Roela, RA, Bordin, NA, Corrêa, LA, Cardoso, JP & De Campos Zuccari, DAP 2016, 'Melatonin regulates angiogenic and inflammatory proteins in MDA-MB-231 cell line and in co-culture with cancer-associated fibroblasts', Mini-Reviews in Medicinal Chemistry, vol. 16, no. 17, pp. 1474-1484. https://doi.org/10.2174/1871520616666160422105920
Maschio-Signorini, Larissa B. ; Gelaleti, Gabriela B. ; Moschetta, Marina G. ; Borin, Thaiz Ferraz ; Jardim-Perassi, Bruna V. ; Lopes, Juliana R. ; Lacerda, Jessica Z. ; Roela, Rosimeire A. ; Bordin, Newton A. ; Corrêa, Lizandra A. ; Cardoso, Jessica P. ; De Campos Zuccari, Debora Aparecida Pires. / Melatonin regulates angiogenic and inflammatory proteins in MDA-MB-231 cell line and in co-culture with cancer-associated fibroblasts. In: Mini-Reviews in Medicinal Chemistry. 2016 ; Vol. 16, No. 17. pp. 1474-1484.
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AU - Gelaleti, Gabriela B.

AU - Moschetta, Marina G.

AU - Borin, Thaiz Ferraz

AU - Jardim-Perassi, Bruna V.

AU - Lopes, Juliana R.

AU - Lacerda, Jessica Z.

AU - Roela, Rosimeire A.

AU - Bordin, Newton A.

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