Membranverletzungen von Pankreasazinuszellen stellen eine der ersten Veränderungen im Frühstadium der akuten experimentellen Pankreatitis dar.

Translated title of the contribution: Membrane injuries of pancreatic acinar cells are the first changes in early stage acute experimental pancreatitis

M. W. Müller, D. E. Bockman, Paul L McNeil, M. Olma, M. W. Büchler, H. G. Beger

Research output: Contribution to journalArticle

1 Scopus citations


We tested the hypothesis that membrane wounding of acinar cells is one of the earliest changes during the induction of acute pancreatitis. Wounding of cell membranes was detected by the penetration of the animals own albumin into cells. The pancreatitis was induced by the intraperitoneal injection of supramaximal doses of caerulein. The controls received saline. Fifteen to 180 min. after the injection the animals were perfused with buffer followed by fixative. Frozen sections of pancreas were processed identically for immunocytological localization of albumin. The intensity of staining was quantified by image analysis. Animals receiving caerulein consistently display significantly greater (p < 0.001) anti-albumin immunostaining in the cytoplasm of acinar cells than controls. The penetration of albumin into acinar cells indicates that wounding of their plasma membrane occurs during the onset of acute pancreatitis. Wounding of membranes may allow the exit of molecules such as enzymes from the acinar cells during this period.

Original languageGerman
Pages (from-to)421-425
Number of pages5
JournalLangenbecks Archiv für Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft für Chirurgie. Kongress
Issue numberSuppl I
StatePublished - Jan 1 1998


ASJC Scopus subject areas

  • Medicine(all)

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