Membrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells

F. Gisou Van Der Goot, Jérôme Pugin, Marusa Hribar, Lucie Fransen, Yves Dunant, Patrick De Baetselier, Alain Bloc, Rudolf Lucas

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Tumor necrosis factor (TNF) can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like wild type TNF. A peptide mimicking the lectin-like domain, which can still trigger increases in membrane currents in cells, failed to interact with liposomes. Thus, the capacity of TNF to trigger increases in membrane conductance in mammalian cells does not correlate with its ability to interact with membranes, suggesting that the cytokine does not form channels itself, but rather interacts with endogenous ion channels or with plasma membrane proteins that are coupled to ion channels. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalFEBS Letters
Volume460
Issue number1
DOIs
StatePublished - Oct 22 1999
Externally publishedYes

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Keywords

  • Channel
  • Membrane
  • Tumor necrosis factor
  • Unfolding

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Van Der Goot, F. G., Pugin, J., Hribar, M., Fransen, L., Dunant, Y., De Baetselier, P., Bloc, A., & Lucas, R. (1999). Membrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells. FEBS Letters, 460(1), 107-111. https://doi.org/10.1016/S0014-5793(99)01294-6