Metabolic responses to leptin in obese db/db mice are strain dependent

Ruth Babette Harris, Tiffany D. Mitchell, Xiaolang Yan, Jacob S. Simpson, Stephen M. Redmann

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Obese, diabetic C57BL/Ks db/db mice that lack the long-form leptin receptor exhibit no decrease in body weight or food intake when treated with leptin. Here we compared responses to leptin in two strains of db/db mice: C57BL/6J mice that are hyperglycemic and hyperinsulinemic and C57BL/Ks that are hyperglycemic and normo- or hypoinsulinemic. Chronic intraperitoneal infusion of 10 μg leptin/day partially reversed hyperglycemia in C57BL/6J male mice but exaggerated the diabetic state of female mice. Bolus intraperitoneal injections of 40 μg leptin/day did not effect glucose in either strain of male db/db mice, whereas chronic intraperitoneal infusion of 20 μg leptin/day significantly reduced fasting blood glucose in male mice from both strains, especially C57BL/6J mice. Food intake, body weight, rectal temperature, and body fat did not change. Chronic intraperitoneal infusion of 10 μg leptin/day significantly reduced body fat in lean db/ + C57BL/6J but not in C57BL/Ks mice. Thus peripherally administered leptin is active in mice that have only short-form leptin receptors, and the response is dependent on the method of leptin administration and the background strain.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number1 50-1
StatePublished - Oct 10 2001
Externally publishedYes

Fingerprint

Leptin
Parenteral Infusions
Inbred C57BL Mouse
Leptin Receptors
Adipose Tissue
Eating
Body Weight
Obese Mice
Intraperitoneal Injections
Hyperglycemia
Blood Glucose
Fasting
Glucose
Temperature

Keywords

  • Body fat
  • Fasting glucose
  • Glucose tolerance

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Metabolic responses to leptin in obese db/db mice are strain dependent. / Harris, Ruth Babette; Mitchell, Tiffany D.; Yan, Xiaolang; Simpson, Jacob S.; Redmann, Stephen M.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 281, No. 1 50-1, 10.10.2001.

Research output: Contribution to journalArticle

Harris, Ruth Babette ; Mitchell, Tiffany D. ; Yan, Xiaolang ; Simpson, Jacob S. ; Redmann, Stephen M. / Metabolic responses to leptin in obese db/db mice are strain dependent. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2001 ; Vol. 281, No. 1 50-1.
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