Metabolism of endothelin-1 by neuroblastoma x glioma hybrid (NG108-15) cells

Krassimira Angelova, Adviye Ergul, Kou Cheng Peng, David Puett

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The endothelin (ET) peptides, ET-1, ET-2, and ET-3, as well as the ET(A) and ET(B) receptor subtypes, are known to occur in brain, but there is a dearth of information on the metabolism of these peptides by the central nervous system (CNS). In this study we have investigated the kinetics of ET-1 binding to and dissociation from the hybrid neuroblastoma x glioma cell line, NG108-15, which is known to contain functional ET receptors, and metabolism of bound ET-1. [125I]ET-1 was incubated with cells for various periods of time up to 6 h, and the nature of the radioactivity in the cell medium and lysate was analyzed by reverse phase high performance liquid chromatography (HPLC). It was found that NG108-15 cells are capable of degrading [125I]ET-1 to [125I]Tyr and several fragments of intermediate hydrophobicity; however, a portion of the cell-associated [125I]ET-1 was protected from degradation for several hours.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalNeuroscience Letters
Volume225
Issue number1
DOIs
StatePublished - Mar 28 1997

    Fingerprint

Keywords

  • Endothelin degradation
  • Endothelin-1
  • NG108-15 cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this