Method of leptin dosing, strain, and group housing influence leptin sensitivity in high-fat-fed weanling mice

Heather Bowen, Tiffany D. Mitchell, Ruth Babette Harris

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

High-fat diets are reported to induce resistance to peripherally administered leptin. In an attempt to develop a model of juvenile diet-induced obesity, mice were weaned onto high-fat diet. Male and female, 35-day-old, C57BL/6J high-fat (45% kcal fat) diet-fed mice housed individually on grid floors did not decrease food intake or body weight in response to intraperitoneal (30 μg), lateral ventricle (5 μg), or third ventricle (0.5 μg) injections of leptin. Body weight and fat were significantly reduced by 13-day intraperitoneal infusions of 10 μg leptin/day, which doubled circulating leptin. Leptin infusion also reduced body fat in weanling, high-fat diet-fed NIH Swiss mice. Group housing mice on bedding prevented loss of fat in high-fat diet-fed male and female NIH Swiss and female C57BL/6J mice. These results indicate that peripherally infused leptin reduces fat in part by increasing thermogenesis and that inhibition of food intake in high-fat diet-fed mice requires either chronic activation of central leptin receptors or is independent of receptors that inhibit feeding in response to an acute central injection of leptin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume284
Issue number1 53-1
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

Leptin
High Fat Diet
Fats
Adipose Tissue
Eating
Body Weight
Parenteral Infusions
Diet
Leptin Receptors
Injections
Third Ventricle
Thermogenesis
Lateral Ventricles
Inbred C57BL Mouse
Obesity

Keywords

  • Body composition
  • Body weight
  • Food intake
  • Serum leptin

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

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