Methotrexate interferes with determinations of conjugated bilirubin with the Kodak Ektachem 400

S. Swanson; T. E. Miffin; J. C. Boyd

doi:10.1093/clinchem/32.5.863

Methotrexate interferes with determinations of conjugated bilirubin with the Kodak Ektachem 400

S. Swanson, T. E. Miffin, J. C. Boyd

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6 Scopus citations

Abstract

Immediately after intravenous infusion of a high dose (concentration in serum > 1000 μmol/L) of methotrexate, the apparent conjugated bilirubin (Bc) concentrations in serum of two osteosarcoma patients, as measured by the Kodak Ektachem 400 analyzer, were greater than the corresponding total bilirubin concentrations, but decreased as the concentrations of methotrexate in serum decreased. In an interference study we found that methotrexate added to sera containing a wide range of basal Bc concentrations increased the measured Bc concentration in a linear and dose-related fashion. Methotrexate also interfered negatively with measurements of unconjugated bilirubin (Bu). The source of the interference appears to be an overlap in the absorption spectrum of methotrexate with Bc and Bu at 400 nm.

Original language	English (US)
Pages (from-to)	863-864
Number of pages	2
Journal	Clinical Chemistry
Volume	32
Issue number	5
DOIs	https://doi.org/10.1093/clinchem/32.5.863
State	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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title = "Methotrexate interferes with determinations of conjugated bilirubin with the Kodak Ektachem 400",

abstract = "Immediately after intravenous infusion of a high dose (concentration in serum > 1000 μmol/L) of methotrexate, the apparent conjugated bilirubin (Bc) concentrations in serum of two osteosarcoma patients, as measured by the Kodak Ektachem 400 analyzer, were greater than the corresponding total bilirubin concentrations, but decreased as the concentrations of methotrexate in serum decreased. In an interference study we found that methotrexate added to sera containing a wide range of basal Bc concentrations increased the measured Bc concentration in a linear and dose-related fashion. Methotrexate also interfered negatively with measurements of unconjugated bilirubin (Bu). The source of the interference appears to be an overlap in the absorption spectrum of methotrexate with Bc and Bu at 400 nm.",

author = "S. Swanson and Miffin, {T. E.} and Boyd, {J. C.}",

year = "1986",

doi = "10.1093/clinchem/32.5.863",

language = "English (US)",

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journal = "Clinical Chemistry",

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TY - JOUR

T1 - Methotrexate interferes with determinations of conjugated bilirubin with the Kodak Ektachem 400

AU - Swanson, S.

AU - Miffin, T. E.

AU - Boyd, J. C.

PY - 1986

Y1 - 1986

N2 - Immediately after intravenous infusion of a high dose (concentration in serum > 1000 μmol/L) of methotrexate, the apparent conjugated bilirubin (Bc) concentrations in serum of two osteosarcoma patients, as measured by the Kodak Ektachem 400 analyzer, were greater than the corresponding total bilirubin concentrations, but decreased as the concentrations of methotrexate in serum decreased. In an interference study we found that methotrexate added to sera containing a wide range of basal Bc concentrations increased the measured Bc concentration in a linear and dose-related fashion. Methotrexate also interfered negatively with measurements of unconjugated bilirubin (Bu). The source of the interference appears to be an overlap in the absorption spectrum of methotrexate with Bc and Bu at 400 nm.

AB - Immediately after intravenous infusion of a high dose (concentration in serum > 1000 μmol/L) of methotrexate, the apparent conjugated bilirubin (Bc) concentrations in serum of two osteosarcoma patients, as measured by the Kodak Ektachem 400 analyzer, were greater than the corresponding total bilirubin concentrations, but decreased as the concentrations of methotrexate in serum decreased. In an interference study we found that methotrexate added to sera containing a wide range of basal Bc concentrations increased the measured Bc concentration in a linear and dose-related fashion. Methotrexate also interfered negatively with measurements of unconjugated bilirubin (Bu). The source of the interference appears to be an overlap in the absorption spectrum of methotrexate with Bc and Bu at 400 nm.

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Methotrexate interferes with determinations of conjugated bilirubin with the Kodak Ektachem 400

Abstract

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