Methyl-β-cyclodextrin prevents angiotensin II-induced tachyphylactic contractile responses in rat aorta

A. Elizabeth Linder, Keshari M. Thakali, Janice M. Thompson, Stephanie W. Watts, R Clinton Webb, Romulo Leite

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Tachyphylaxis or desensitization is frequently observed following angiotensin II type I (AT1) receptor activation by angiotensin II. One of the possible mechanisms contributing to receptor desensitization involves receptor internalization. In addition to clathrin-coated pits/vesicles, caveolae, small invaginations in the plasma membrane rich in cholesterol, may also be involved in receptor internalization. After activation, AT1 receptor partially redistributes to lipid-enriched domains. We hypothesize that AT1 receptor internalization via caveolae contributes to the tachyphylactic response observed to angiotensin II. Endothelium-denuded rat aortic rings were exposed to increasing concentrations of angiotensin II or phenylephrine, generating two cumulative concentration-effect curves (CCEC) with a 90-min interval separating each curve (CCEC-I and CCEC-II). CCEC-II was performed in the presence of either vehicle or methyl-β-cyclodextrin (CD), a drug that depletes cholesterol from the membrane and disassembles caveolae. CCEC-II to angiotensin II, but not to phenylephrine, was blunted in aortic rings treated with vehicle. In the presence of CD, CCEC-II did not differ significantly from CCEC-I for both agonists. CCEC-I to angiotensin II was abolished when in the presence of the AT1 receptor antagonist. The presence of AT1 receptors at the aortic smooth muscle cells' membrane treated with angiotensin II was observed by immunofluorescence only in the presence of CD. In addition, caveolin-1 coimmunoprecipitated with AT1 receptor after agonist stimulation, and this interaction was inhibited by CD. Our data suggest that caveolae are involved in the tachyphylactic contractile response induced by angiotensin II in rat aorta, and this effect is related to receptor internalization.

Original languageEnglish (US)
Pages (from-to)78-84
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume323
Issue number1
DOIs
StatePublished - Oct 1 2007

Fingerprint

Cyclodextrins
Angiotensin II
Aorta
Caveolae
Phenylephrine
Cholesterol
Cell Membrane
Clathrin-Coated Vesicles
Tachyphylaxis
Caveolin 1
Angiotensin I
Angiotensin Type 1 Receptor
Angiotensin Receptors
Smooth Muscle Myocytes
Endothelium
Fluorescent Antibody Technique
Lipids
Membranes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Methyl-β-cyclodextrin prevents angiotensin II-induced tachyphylactic contractile responses in rat aorta. / Linder, A. Elizabeth; Thakali, Keshari M.; Thompson, Janice M.; Watts, Stephanie W.; Webb, R Clinton; Leite, Romulo.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 323, No. 1, 01.10.2007, p. 78-84.

Research output: Contribution to journalArticle

Linder, A. Elizabeth ; Thakali, Keshari M. ; Thompson, Janice M. ; Watts, Stephanie W. ; Webb, R Clinton ; Leite, Romulo. / Methyl-β-cyclodextrin prevents angiotensin II-induced tachyphylactic contractile responses in rat aorta. In: Journal of Pharmacology and Experimental Therapeutics. 2007 ; Vol. 323, No. 1. pp. 78-84.
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