MG2 and lactoferrin form a heterotypic complex in salivary secretions

R. V. Soares, Camille Colares Siqueira Nogueira, L. S. Bruno, F. G. Oppenheim, G. D. Offner, R. F. Troxler

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Protein-protein interactions are necessary for homeostasis to be maintained and for biological systems to be integrated. Heterotypic complexes occur in saliva, and a complex between MG2 and SIgA has been suggested to promote microbial clearance from the oral cavity. In this study, we used a peptide display library to investigate previously unrecognized heterotypic complexes involving MG2 and other proteins. The library was panned with MG2 12 times, and analyses of clones identified the sequence Ala-Leu-Leu-Cys-, which occurs in salivary lactoferrin. Blotting experiments confirmed that MG2 and lactoferrin form a heterotypic complex in viro and in vivo. Periodate treatment of MG2 did not affect the interaction. A synthetic lactoferrin peptide containing the motif Ala-Leu-Leu-Cys- blocked the interaction between MG2 and lactoferrin, confirming the specificity of the interaction identified by panning. This complex may enhance the properties of these salivary components in the oral environment.

Original languageEnglish (US)
Pages (from-to)471-475
Number of pages5
JournalJournal of Dental Research
Volume82
Issue number6
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

Lactoferrin
Peptide Library
Secretory Immunoglobulin A
Proteins
Saliva
Libraries
Mouth
Homeostasis
Clone Cells
Peptides

Keywords

  • Protein-protein interactions
  • Saliva
  • Salivary proteins

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

MG2 and lactoferrin form a heterotypic complex in salivary secretions. / Soares, R. V.; Siqueira Nogueira, Camille Colares; Bruno, L. S.; Oppenheim, F. G.; Offner, G. D.; Troxler, R. F.

In: Journal of Dental Research, Vol. 82, No. 6, 01.01.2003, p. 471-475.

Research output: Contribution to journalArticle

Soares, R. V. ; Siqueira Nogueira, Camille Colares ; Bruno, L. S. ; Oppenheim, F. G. ; Offner, G. D. ; Troxler, R. F. / MG2 and lactoferrin form a heterotypic complex in salivary secretions. In: Journal of Dental Research. 2003 ; Vol. 82, No. 6. pp. 471-475.
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