Microarray expression profiling in adhesion and normal peritoneal tissues

Dana R. Ambler, Alicia M. Golden, Jennifer S. Gell, Ghassan M. Saed, David J. Carey, Michael Peter Diamond

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: To identify molecular markers associated with adhesion and normal peritoneal tissue using microarray expression profiling. Design: Comparative study. Setting: University hospital. Patient(s): Five premenopausal women. Intervention(s): Adhesion and normal peritoneal tissue samples were obtained from premenopausal women. Ribonucleic acid was extracted using standard protocols and processed for hybridization to Affymetrix Whole Transcript Human Gene Expression Chips. Microarray data were obtained from five different patients, each with adhesion tissue and normal peritoneal samples. Real-time polymerase chain reaction was performed for confirmation using standard protocols. Main Outcome Measure(s): Gene expression in postoperative adhesion and normal peritoneal tissues. Result(s): A total of 1,263 genes were differentially expressed between adhesion and normal tissues. One hundred seventy-three genes were found to be up-regulated and 56 genes were down-regulated in the adhesion tissues compared with normal peritoneal tissues. The genes were sorted into functional categories according to Gene Ontology annotations. Twenty-six up-regulated genes and 11 down-regulated genes were identified with functions potentially relevant to the pathophysiology of postoperative adhesions. We evaluated and confirmed expression of 12 of these specific genes via polymerase chain reaction. Conclusion(s): The pathogenesis, natural history, and optimal treatment of postoperative adhesive disease remains unanswered. Microarray analysis of adhesions identified specific genes with increased and decreased expression when compared with normal peritoneum. Knowledge of these genes and ontologic pathways with altered expression provide targets for new therapies to treat patients who have or are at risk for postoperative adhesions.

Original languageEnglish (US)
JournalFertility and Sterility
Volume97
Issue number5
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Fingerprint

Tissue Adhesions
Genes
Molecular Sequence Annotation
Gene Expression
Gene Ontology
Peritoneum
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Natural History
Adhesives
Real-Time Polymerase Chain Reaction
Outcome Assessment (Health Care)
RNA
Polymerase Chain Reaction
Therapeutics

Keywords

  • Postoperative adhesions
  • gene expression
  • microarray
  • peritoneum

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Microarray expression profiling in adhesion and normal peritoneal tissues. / Ambler, Dana R.; Golden, Alicia M.; Gell, Jennifer S.; Saed, Ghassan M.; Carey, David J.; Diamond, Michael Peter.

In: Fertility and Sterility, Vol. 97, No. 5, 01.01.2012.

Research output: Contribution to journalArticle

Ambler, Dana R. ; Golden, Alicia M. ; Gell, Jennifer S. ; Saed, Ghassan M. ; Carey, David J. ; Diamond, Michael Peter. / Microarray expression profiling in adhesion and normal peritoneal tissues. In: Fertility and Sterility. 2012 ; Vol. 97, No. 5.
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