Microbiologic effect of two topical anti-infective treatments on ligature-induced peri-implantitis

A pilot study in dogs

Umberto Demoner Ramos, Flavia Suaid, Ulf Me Wikesjo, Cristiano Susin, Patrícia Conde Vital, Sérgio Luis Scombatti de Souza, Michel Reis Messora, Daniela Bazan Palioto, Arthur Belém Novaes

Research output: Contribution to journalArticle

Abstract

Background: The aim of this split-mouth design pilot study in dogs was to assess microbiologic effects of two topical anti-infective treatment protocols on dental implants subjected to ligature-induced peri-implantitis, without use of systemic antibiotics. Methods: Eight adult Beagle dogs each received four dental implants in contralateral, edentulated, mandibular jaw quadrants. After 8 weeks, silk ligatures were installed, to be removed after another 8 weeks. After 6 additional weeks, induced peri-implantitis lesions were subjected to either antimicrobial photodynamic therapy (aPDT) or a topical tetracycline (TTC) hydrochloride (50 mg/mL) solution. Microbiologic samples were collected from the deepest proximal peri-implantitis site in each jaw quadrant before and after treatment. The samples were analyzed using DNA-DNA hybridization checkerboard technique. Results: Peri-implantitis induction successfully produced lesions with microbiologic characteristics similar to those found in humans. Overall results showed effective bacterial count reductions for both protocols. aPDT demonstrated major reductions of the red complex, but no statistical differences between groups were observed when adjusted for multiple comparisons. Conclusion: aPDT and TTC successfully decontaminated infected implant surfaces. Implant decontamination with aPDT appears to be a viable alternative to TTC in the management of peri-implantitis infection.

Original languageEnglish (US)
Pages (from-to)995-1002
Number of pages8
JournalJournal of periodontology
Volume89
Issue number8
DOIs
StatePublished - Jan 1 2018

Fingerprint

Peri-Implantitis
Ligation
Photochemotherapy
Dogs
Tetracycline
Dental Implants
Jaw
Therapeutics
Silk
Decontamination
Bacterial Load
DNA
Clinical Protocols
Mouth
Anti-Bacterial Agents
Infection

Keywords

  • Antimicrobial photodynamic therapy
  • Checkerboard
  • Peri-implantitis
  • Tetracycline

ASJC Scopus subject areas

  • Periodontics

Cite this

Ramos, U. D., Suaid, F., Wikesjo, U. M., Susin, C., Vital, P. C., de Souza, S. L. S., ... Novaes, A. B. (2018). Microbiologic effect of two topical anti-infective treatments on ligature-induced peri-implantitis: A pilot study in dogs. Journal of periodontology, 89(8), 995-1002. https://doi.org/10.1002/JPER.17-0630

Microbiologic effect of two topical anti-infective treatments on ligature-induced peri-implantitis : A pilot study in dogs. / Ramos, Umberto Demoner; Suaid, Flavia; Wikesjo, Ulf Me; Susin, Cristiano; Vital, Patrícia Conde; de Souza, Sérgio Luis Scombatti; Messora, Michel Reis; Palioto, Daniela Bazan; Novaes, Arthur Belém.

In: Journal of periodontology, Vol. 89, No. 8, 01.01.2018, p. 995-1002.

Research output: Contribution to journalArticle

Ramos, UD, Suaid, F, Wikesjo, UM, Susin, C, Vital, PC, de Souza, SLS, Messora, MR, Palioto, DB & Novaes, AB 2018, 'Microbiologic effect of two topical anti-infective treatments on ligature-induced peri-implantitis: A pilot study in dogs', Journal of periodontology, vol. 89, no. 8, pp. 995-1002. https://doi.org/10.1002/JPER.17-0630
Ramos, Umberto Demoner ; Suaid, Flavia ; Wikesjo, Ulf Me ; Susin, Cristiano ; Vital, Patrícia Conde ; de Souza, Sérgio Luis Scombatti ; Messora, Michel Reis ; Palioto, Daniela Bazan ; Novaes, Arthur Belém. / Microbiologic effect of two topical anti-infective treatments on ligature-induced peri-implantitis : A pilot study in dogs. In: Journal of periodontology. 2018 ; Vol. 89, No. 8. pp. 995-1002.
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