Micromorphological changes in resin-dentin bonds after 1 year of water storage

Masanori Hashimoto, Hiroki Ohno, Hidehiko Sano, Franklin Chi Meng Tay, Masayuki Kaga, Yoshiyuki Kudou, Haruhisa Oguchi, Yoshima Araki, Minoru Kubota

Research output: Contribution to journalArticle

196 Scopus citations


The purpose of this study was to evaluate the degradation of resin-dentin bonds after 1 year of water storage. Resin-dentin-bonded specimens were prepared with the use of an adhesive resin system (One-Step: Bisco). Half of the experimental specimens were sectioned perpendicular to the adhesive interface to produce a beam (adhesive area: 0.9 mm2) before being stored in distilled water at 37°C for 1 year. The remaining half of the bonded specimens were sectioned into beams of similar dimensions after 1 year of water storage. Additional bonded specimens that had been stored in water for 24 h before sectioning into beams were used as controls. The beams in the two experimental groups and the control group were subjected to microtensile bond testing. Fractography was performed on all fractured beams with the use of FE-SEM. There were significant (p < .05) differences in bond strength among the control specimens (55.9 ± 12.9 MPa), specimens that had been sectioned into beams after water storage (68.9 ± 18.6 MPa), and specimens that had been sectioned into beams before water storage (28.1 ± 9.3 MPa). Fractography revealed that the resin material was gradually extracted from the periphery to the center portion of the beam. This probably accounted for the decrease in bond strength after 1 year of water storage.

Original languageEnglish (US)
Pages (from-to)306-311
Number of pages6
JournalJournal of Biomedical Materials Research
Issue number3
Publication statusPublished - May 27 2002
Externally publishedYes



  • Degradation
  • Fractographic analysis
  • Hybrid layer
  • Microtensile bond test
  • Water storage testing

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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