Micromorphology of resin-dentin interfaces using one-bottle etch&rinse and self-etching adhesive systems on laser-treated dentin surfaces: A confocal laser scanning microscope analysis

Marcelo Tavares De Oliveira, Cesar Augusto Galvão Arrais, Ana Cecília Aranha, Carlos De Paula Eduardo, Katsuya Miyake, Frederick Rueggeberg, Marcelo Giannini

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three adhesive systems (AS) on dentin surfaces treated with Er:YAG laser using two irradiation parameters. Study Design: Occlusal flat dentin surfaces of 36 human third molars were assigned into nine groups (n=4) according to the following ASs: one bottle etch&rinse Single Bond Plus (3M ESPE), two-step Clearfil Protect Bond (Kuraray), and all-in-one S3 Bond (Kuraray) self-etching, which were labeled with rhodamine B or fluorescein isothiocyanate-dextran and were applied to dentin surfaces that were irradiated with Er:YAG laser at either 120 (38.7 J/cm2) or 200 mJ/pulse (64.5 J/cm2), or were applied to untreated dentin surfaces (control group). The ASs were light-activated following MI and the bonded surfaces were restored with resin composite Z250 (3M ESPE). After 24 hours of storage in vegetable oil, the restored teeth were vertically, serially sectioned into 1-mm thick slabs, which had the adhesive interfaces analyzed with confocal laser microscope (CLSM - LSM 510 Meta). CLSM images were recorded in the fluorescent mode from three different regions along each bonded interface. Results: Non-uniform HL was created on laser-irradiated dentin surfaces regardless of laser irradiation protocol for all AS, while regular and uniform HL was observed in the control groups. "Stretch mark"-like red lines were found within the HL as a result of resin infiltration into dentin microfissures, which were predominantly observed in 200 mJ/pulse groups regardless of AS. Poor resin infiltration into peritubular dentin was observed in most regions of adhesive interfaces created by all ASs on laser-irradiated dentin, resulting in thin resin tags with neither funnelshaped morphology nor lateral resin projections. Conclusion: Laser irradiation of dentin surfaces at 120 or 200 mJ/pulse resulted in morphological changes in HL and resin tags for all ASs evaluated in the study.

Original languageEnglish (US)
Pages (from-to)662-670
Number of pages9
JournalLasers in Surgery and Medicine
Volume42
Issue number7
DOIs
StatePublished - Sep 1 2010

Fingerprint

Dentin
Adhesives
Lasers
rhodamine B
Solid-State Lasers
Striae Distensae
Control Groups
Third Molar
Plant Oils
Composite Resins
Tooth
Light

Keywords

  • Bonding agents
  • Bonding interface morphology
  • Dentin
  • Er:YAG laser

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Micromorphology of resin-dentin interfaces using one-bottle etch&rinse and self-etching adhesive systems on laser-treated dentin surfaces : A confocal laser scanning microscope analysis. / De Oliveira, Marcelo Tavares; Arrais, Cesar Augusto Galvão; Aranha, Ana Cecília; Eduardo, Carlos De Paula; Miyake, Katsuya; Rueggeberg, Frederick; Giannini, Marcelo.

In: Lasers in Surgery and Medicine, Vol. 42, No. 7, 01.09.2010, p. 662-670.

Research output: Contribution to journalArticle

De Oliveira, Marcelo Tavares ; Arrais, Cesar Augusto Galvão ; Aranha, Ana Cecília ; Eduardo, Carlos De Paula ; Miyake, Katsuya ; Rueggeberg, Frederick ; Giannini, Marcelo. / Micromorphology of resin-dentin interfaces using one-bottle etch&rinse and self-etching adhesive systems on laser-treated dentin surfaces : A confocal laser scanning microscope analysis. In: Lasers in Surgery and Medicine. 2010 ; Vol. 42, No. 7. pp. 662-670.
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abstract = "Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three adhesive systems (AS) on dentin surfaces treated with Er:YAG laser using two irradiation parameters. Study Design: Occlusal flat dentin surfaces of 36 human third molars were assigned into nine groups (n=4) according to the following ASs: one bottle etch&rinse Single Bond Plus (3M ESPE), two-step Clearfil Protect Bond (Kuraray), and all-in-one S3 Bond (Kuraray) self-etching, which were labeled with rhodamine B or fluorescein isothiocyanate-dextran and were applied to dentin surfaces that were irradiated with Er:YAG laser at either 120 (38.7 J/cm2) or 200 mJ/pulse (64.5 J/cm2), or were applied to untreated dentin surfaces (control group). The ASs were light-activated following MI and the bonded surfaces were restored with resin composite Z250 (3M ESPE). After 24 hours of storage in vegetable oil, the restored teeth were vertically, serially sectioned into 1-mm thick slabs, which had the adhesive interfaces analyzed with confocal laser microscope (CLSM - LSM 510 Meta). CLSM images were recorded in the fluorescent mode from three different regions along each bonded interface. Results: Non-uniform HL was created on laser-irradiated dentin surfaces regardless of laser irradiation protocol for all AS, while regular and uniform HL was observed in the control groups. {"}Stretch mark{"}-like red lines were found within the HL as a result of resin infiltration into dentin microfissures, which were predominantly observed in 200 mJ/pulse groups regardless of AS. Poor resin infiltration into peritubular dentin was observed in most regions of adhesive interfaces created by all ASs on laser-irradiated dentin, resulting in thin resin tags with neither funnelshaped morphology nor lateral resin projections. Conclusion: Laser irradiation of dentin surfaces at 120 or 200 mJ/pulse resulted in morphological changes in HL and resin tags for all ASs evaluated in the study.",
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T2 - A confocal laser scanning microscope analysis

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AU - Arrais, Cesar Augusto Galvão

AU - Aranha, Ana Cecília

AU - Eduardo, Carlos De Paula

AU - Miyake, Katsuya

AU - Rueggeberg, Frederick

AU - Giannini, Marcelo

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N2 - Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three adhesive systems (AS) on dentin surfaces treated with Er:YAG laser using two irradiation parameters. Study Design: Occlusal flat dentin surfaces of 36 human third molars were assigned into nine groups (n=4) according to the following ASs: one bottle etch&rinse Single Bond Plus (3M ESPE), two-step Clearfil Protect Bond (Kuraray), and all-in-one S3 Bond (Kuraray) self-etching, which were labeled with rhodamine B or fluorescein isothiocyanate-dextran and were applied to dentin surfaces that were irradiated with Er:YAG laser at either 120 (38.7 J/cm2) or 200 mJ/pulse (64.5 J/cm2), or were applied to untreated dentin surfaces (control group). The ASs were light-activated following MI and the bonded surfaces were restored with resin composite Z250 (3M ESPE). After 24 hours of storage in vegetable oil, the restored teeth were vertically, serially sectioned into 1-mm thick slabs, which had the adhesive interfaces analyzed with confocal laser microscope (CLSM - LSM 510 Meta). CLSM images were recorded in the fluorescent mode from three different regions along each bonded interface. Results: Non-uniform HL was created on laser-irradiated dentin surfaces regardless of laser irradiation protocol for all AS, while regular and uniform HL was observed in the control groups. "Stretch mark"-like red lines were found within the HL as a result of resin infiltration into dentin microfissures, which were predominantly observed in 200 mJ/pulse groups regardless of AS. Poor resin infiltration into peritubular dentin was observed in most regions of adhesive interfaces created by all ASs on laser-irradiated dentin, resulting in thin resin tags with neither funnelshaped morphology nor lateral resin projections. Conclusion: Laser irradiation of dentin surfaces at 120 or 200 mJ/pulse resulted in morphological changes in HL and resin tags for all ASs evaluated in the study.

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KW - Bonding agents

KW - Bonding interface morphology

KW - Dentin

KW - Er:YAG laser

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