Micromorphology of resin/dentin interfaces using 4th and 5th generation dual-curing adhesive/cement systems: A confocal laser scanning microscope analysis

Cesar A.G. Arrais, Katsuia Miyake, Frederick Rueggeberg, David H. Pashley, Marcelo Giannini

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: This study evaluated the differential composition of resin/dentin interfaces of indirect restorations created by the application of 4th and 5th generation dual-curing luting systems (bonding agents/resin cements), when each material was either light cured or allowed to self-cure. Materials and Methods: Occlusal flat dentin surfaces of 60 human third molars were assigned into 12 groups (n = 5) according to curing mode and dual-curing cementing system: 4th generation All Bond2 (AB2)/Duolink (Bisco) and 5th generation (B1) Bond1/Lute-it (Pentron). Fluorescein-labeled dextran (FDx) was mixed with the bonding agents, while rhodamine-labeled dextran (RhDx) was incorporated into resin cements and Pre-Bond resin from AB2. Resin cements were applied to 2-mm-thick, precured resin composite disks (Z250, 3M ESPE), which were fixed to dentin surfaces containing adhesive resin in either cured (light cured; LC) or uncured (self-cured; SC) states. The restored teeth were light activated (XL3000, 3M ESPE) according to the manufacturers’ instructions (LRC) or allowed to self-cure (SRC), were stored for 24 h, and then vertically, serially sectioned into 1-mm-thick slabs, which were analyzed using confocal laser scanning microscopy. Fluorescent additives indicated where individual components of the bonding/cement systems were located. Additional specimens were prepared and analyzed using a conventional scanning electron microscope. Results: AB2/LC and B1/LC exhibited nonuniform primer/adhesive layer thickness. AB2/SC showed adhesive resin penetration within the primed dentin, and resin cement penetration at the entrance of the dentin tubules. B1/SC/LRC demonstrated resin cement penetration within the hybrid layer and into the dentin tubules. More resin cement penetration was observed in B1/SC/SRC groups than in its LRC equivalent. Conclusion: The morphological features and component interactions among materials at resin/dentin interfaces are related to the activation modes of the primer/adhesive layer and of the resin cement used.

Original languageEnglish (US)
Pages (from-to)15-26
Number of pages12
JournalJournal of Adhesive Dentistry
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2009

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Resin Cements
Dentin
Lasers
Adhesives
Light
Third Molar
Composite Resins
Adhesive cement
Confocal Microscopy
Tooth
Electrons

Keywords

  • Adhesive interfacial morphology
  • Confocal laser scanning microscopy
  • Dual-curing adhesive systems
  • Dual-curing resin cements
  • Indirect restorations

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Micromorphology of resin/dentin interfaces using 4th and 5th generation dual-curing adhesive/cement systems : A confocal laser scanning microscope analysis. / Arrais, Cesar A.G.; Miyake, Katsuia; Rueggeberg, Frederick; Pashley, David H.; Giannini, Marcelo.

In: Journal of Adhesive Dentistry, Vol. 11, No. 1, 01.01.2009, p. 15-26.

Research output: Contribution to journalArticle

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abstract = "Purpose: This study evaluated the differential composition of resin/dentin interfaces of indirect restorations created by the application of 4th and 5th generation dual-curing luting systems (bonding agents/resin cements), when each material was either light cured or allowed to self-cure. Materials and Methods: Occlusal flat dentin surfaces of 60 human third molars were assigned into 12 groups (n = 5) according to curing mode and dual-curing cementing system: 4th generation All Bond2 (AB2)/Duolink (Bisco) and 5th generation (B1) Bond1/Lute-it (Pentron). Fluorescein-labeled dextran (FDx) was mixed with the bonding agents, while rhodamine-labeled dextran (RhDx) was incorporated into resin cements and Pre-Bond resin from AB2. Resin cements were applied to 2-mm-thick, precured resin composite disks (Z250, 3M ESPE), which were fixed to dentin surfaces containing adhesive resin in either cured (light cured; LC) or uncured (self-cured; SC) states. The restored teeth were light activated (XL3000, 3M ESPE) according to the manufacturers’ instructions (LRC) or allowed to self-cure (SRC), were stored for 24 h, and then vertically, serially sectioned into 1-mm-thick slabs, which were analyzed using confocal laser scanning microscopy. Fluorescent additives indicated where individual components of the bonding/cement systems were located. Additional specimens were prepared and analyzed using a conventional scanning electron microscope. Results: AB2/LC and B1/LC exhibited nonuniform primer/adhesive layer thickness. AB2/SC showed adhesive resin penetration within the primed dentin, and resin cement penetration at the entrance of the dentin tubules. B1/SC/LRC demonstrated resin cement penetration within the hybrid layer and into the dentin tubules. More resin cement penetration was observed in B1/SC/SRC groups than in its LRC equivalent. Conclusion: The morphological features and component interactions among materials at resin/dentin interfaces are related to the activation modes of the primer/adhesive layer and of the resin cement used.",
keywords = "Adhesive interfacial morphology, Confocal laser scanning microscopy, Dual-curing adhesive systems, Dual-curing resin cements, Indirect restorations",
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AU - Pashley, David H.

AU - Giannini, Marcelo

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N2 - Purpose: This study evaluated the differential composition of resin/dentin interfaces of indirect restorations created by the application of 4th and 5th generation dual-curing luting systems (bonding agents/resin cements), when each material was either light cured or allowed to self-cure. Materials and Methods: Occlusal flat dentin surfaces of 60 human third molars were assigned into 12 groups (n = 5) according to curing mode and dual-curing cementing system: 4th generation All Bond2 (AB2)/Duolink (Bisco) and 5th generation (B1) Bond1/Lute-it (Pentron). Fluorescein-labeled dextran (FDx) was mixed with the bonding agents, while rhodamine-labeled dextran (RhDx) was incorporated into resin cements and Pre-Bond resin from AB2. Resin cements were applied to 2-mm-thick, precured resin composite disks (Z250, 3M ESPE), which were fixed to dentin surfaces containing adhesive resin in either cured (light cured; LC) or uncured (self-cured; SC) states. The restored teeth were light activated (XL3000, 3M ESPE) according to the manufacturers’ instructions (LRC) or allowed to self-cure (SRC), were stored for 24 h, and then vertically, serially sectioned into 1-mm-thick slabs, which were analyzed using confocal laser scanning microscopy. Fluorescent additives indicated where individual components of the bonding/cement systems were located. Additional specimens were prepared and analyzed using a conventional scanning electron microscope. Results: AB2/LC and B1/LC exhibited nonuniform primer/adhesive layer thickness. AB2/SC showed adhesive resin penetration within the primed dentin, and resin cement penetration at the entrance of the dentin tubules. B1/SC/LRC demonstrated resin cement penetration within the hybrid layer and into the dentin tubules. More resin cement penetration was observed in B1/SC/SRC groups than in its LRC equivalent. Conclusion: The morphological features and component interactions among materials at resin/dentin interfaces are related to the activation modes of the primer/adhesive layer and of the resin cement used.

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KW - Dual-curing resin cements

KW - Indirect restorations

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