MicroRNA-1 regulates the differentiation of adipose-derived stem cells into cardiomyocyte-like cells

Can Chen, Quanxiang Yan, Yiguang Yan, Mudi Ma, Yuan He, Xiaorong Shui, Zhigang Yang, Xiaozhong Lan, Yao Liang Tang, Wei Lei

Research output: Contribution to journalArticle

Abstract

Stem cell transplantation is one of most valuable methods in the treatment of myocardial infarction, and adipose-derived stem cells (ASCs) are becoming a hot topic in medical research. Previous studies have shown that ASCs can be differentiated into cardiomyocyte-like cells, but the efficiency and survival rates are low. We investigated the role and mechanism of microRNA-1 (miR-1) in the differentiation of ASCs into cardiomyocyte-like cells. ASCs and cardiomyocytes were isolated from neonatal rats. We constructed lentivirus for overexpressing miR-1 and used DAPT, an antagonist of the Notch1 pathway, for in vitro analyses. We performed cocultures with ASCs and cardiomyocytes. The differentiation efficiency of ASCs was detected by cell-specific surface antigens. Our results showed that miR-1 can promote the expression of Notch1 and reduce the expression of Hes1, a Notch pathway factor, and overexpression of miR-1 can promote the differentiation of ASCs into cardiomyocyte-like cells, which may occur by regulating Notch1 and Hes1.

Original languageEnglish (US)
Article number7494530
JournalStem Cells International
Volume2018
DOIs
StatePublished - Jan 1 2018

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MicroRNAs
Cardiac Myocytes
Stem Cells
Lentivirus
Stem Cell Transplantation
Surface Antigens
Coculture Techniques
Biomedical Research
Cell Survival
Myocardial Infarction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

MicroRNA-1 regulates the differentiation of adipose-derived stem cells into cardiomyocyte-like cells. / Chen, Can; Yan, Quanxiang; Yan, Yiguang; Ma, Mudi; He, Yuan; Shui, Xiaorong; Yang, Zhigang; Lan, Xiaozhong; Tang, Yao Liang; Lei, Wei.

In: Stem Cells International, Vol. 2018, 7494530, 01.01.2018.

Research output: Contribution to journalArticle

Chen, Can ; Yan, Quanxiang ; Yan, Yiguang ; Ma, Mudi ; He, Yuan ; Shui, Xiaorong ; Yang, Zhigang ; Lan, Xiaozhong ; Tang, Yao Liang ; Lei, Wei. / MicroRNA-1 regulates the differentiation of adipose-derived stem cells into cardiomyocyte-like cells. In: Stem Cells International. 2018 ; Vol. 2018.
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AU - Shui, Xiaorong

AU - Yang, Zhigang

AU - Lan, Xiaozhong

AU - Tang, Yao Liang

AU - Lei, Wei

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