MicroRNA-1 regulates the differentiation of adipose-derived stem cells into cardiomyocyte-like cells

Can Chen, Quanxiang Yan, Yiguang Yan, Mudi Ma, Yuan He, Xiaorong Shui, Zhigang Yang, Xiaozhong Lan, Yao Liang Tang, Wei Lei

Research output: Contribution to journalArticle

Abstract

Stem cell transplantation is one of most valuable methods in the treatment of myocardial infarction, and adipose-derived stem cells (ASCs) are becoming a hot topic in medical research. Previous studies have shown that ASCs can be differentiated into cardiomyocyte-like cells, but the efficiency and survival rates are low. We investigated the role and mechanism of microRNA-1 (miR-1) in the differentiation of ASCs into cardiomyocyte-like cells. ASCs and cardiomyocytes were isolated from neonatal rats. We constructed lentivirus for overexpressing miR-1 and used DAPT, an antagonist of the Notch1 pathway, for in vitro analyses. We performed cocultures with ASCs and cardiomyocytes. The differentiation efficiency of ASCs was detected by cell-specific surface antigens. Our results showed that miR-1 can promote the expression of Notch1 and reduce the expression of Hes1, a Notch pathway factor, and overexpression of miR-1 can promote the differentiation of ASCs into cardiomyocyte-like cells, which may occur by regulating Notch1 and Hes1.

Original languageEnglish (US)
Article number7494530
JournalStem Cells International
Volume2018
DOIs
StatePublished - Jan 1 2018

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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