MicroRNA-145, a novel smooth muscle cell phenotypic marker and modulator, controls vascular neointimal lesion formation

Yunhui Cheng, Xiaojun Liu, Jian Yang, Ying Lin, Da Zhong Xu, Qi Lu, Edwin A. Deitch, Yuqing Huo, Ellise S. Delphin, Chunxiang Zhang

Research output: Contribution to journalArticle

455 Citations (Scopus)

Abstract

Phenotypic modulation of vascular smooth muscle cells (VSMCs) plays a critical role in the pathogenesis of a variety of proliferative vascular diseases. Recently, we have found that microRNA (miRNA) miR-145 is the most abundant miRNA in normal vascular walls and in freshly isolated VSMCs; however, the role of miR-145 in VSMC phenotypic modulation and vascular diseases is currently unknown. Here we find that miR-145 is selectively expressed in VSMCs of the vascular wall and its expression is significantly downregulated in the vascular walls with neointimal lesion formation and in cultured dedifferentiated VSMCs. More importantly, both in cultured rat VSMCs in vitro and in balloon-injured rat carotid arteries in vivo, we demonstrate that the noncoding RNA miR-145 is a novel phenotypic marker and a novel phenotypic modulator of VSMCs. VSMC differentiation marker genes such as SM α-actin, calponin, and SM-MHC are upregulated by premiR-145 or adenovirus expressing miR-145 (Ad-miR-145) but are downregulated by the miR-145 inhibitor 2′OMe-miR-145. We have further identified that miR-145-mediated phenotypic modulation of VSMCs is through its target gene KLF5 and its downstream signaling molecule, myocardin. Finally, restoration of miR-145 in balloon-injured arteries via Ad-miR-145 inhibits neointimal growth. We conclude that miR-145 is a novel VSMC phenotypic marker and modulator that is able of controlling vascular neointimal lesion formation. These novel findings may have extensive implications for the diagnosis and therapy of a variety of proliferative vascular diseases.

Original languageEnglish (US)
Pages (from-to)158-166
Number of pages9
JournalCirculation research
Volume105
Issue number2
DOIs
StatePublished - Jul 17 2009

Fingerprint

MicroRNAs
Vascular Smooth Muscle
Smooth Muscle Myocytes
Blood Vessels
Vascular Diseases
Adenoviridae
Down-Regulation
Untranslated RNA
Differentiation Antigens
Carotid Arteries
Cell Wall
Genes
Actins
Cell Differentiation
Arteries
Growth

Keywords

  • MicroRNAs
  • Phenotype
  • Vascular disease
  • Vascular smooth muscle cells

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

MicroRNA-145, a novel smooth muscle cell phenotypic marker and modulator, controls vascular neointimal lesion formation. / Cheng, Yunhui; Liu, Xiaojun; Yang, Jian; Lin, Ying; Xu, Da Zhong; Lu, Qi; Deitch, Edwin A.; Huo, Yuqing; Delphin, Ellise S.; Zhang, Chunxiang.

In: Circulation research, Vol. 105, No. 2, 17.07.2009, p. 158-166.

Research output: Contribution to journalArticle

Cheng, Yunhui ; Liu, Xiaojun ; Yang, Jian ; Lin, Ying ; Xu, Da Zhong ; Lu, Qi ; Deitch, Edwin A. ; Huo, Yuqing ; Delphin, Ellise S. ; Zhang, Chunxiang. / MicroRNA-145, a novel smooth muscle cell phenotypic marker and modulator, controls vascular neointimal lesion formation. In: Circulation research. 2009 ; Vol. 105, No. 2. pp. 158-166.
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AU - Lu, Qi

AU - Deitch, Edwin A.

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