MicroRNA 155 regulates Japanese encephalitis virus-induced inflammatory response by targeting Src homology 2-containing inositol phosphatase 1

Menaka Thounaojam, Kiran Kundu, Deepak Kumar Kaushik, Shalini Swaroop, Anita Mahadevan, Susarla Krishna Shankar, Anirban Basu

Research output: Contribution to journalArticle

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Abstract

MicroRNAs (miRNAs) are single-stranded small RNA molecules that regulate various cellular processes. miRNA 155 (miR-155) regulates various aspects of innate and adaptive immune responses and plays a key role in various viral infections and the resulting neuroinflammation. The present study evaluated the involvement of miR-155 in modulating Japanese encephalitis virus (JEV)-induced neuroinflammation. We observed that miR-155 expression was upregulated during JEV infection of mouse primary microglia, the BV-2 microglia cell line, and in both mouse and human brains. In vitro and in vivo knockdown of miR-155 minimized JEV-induced inflammatory responses. In the present study, we confirmed targeting of the Src homology 2-containing inositol phosphatase 1 (SHIP1) 3' untranslated region (UTR) by miR-155 in the context of JEV infection. Inhibition of SHIP1 by miR-155 resulted in higher beta interferon (IFN-β) and proinflammatory cytokine production through activation of TANKbinding kinase 1 (TBK-1). Based on these observations, we conclude that miR-155 modulates the neuroinflammatory response during JEV infection via negative regulation of SHIP1 expression. Thus, modulation of miR-155 could be a novel strategy to regulate JEV-induced neuroinflammation.

Original languageEnglish (US)
Pages (from-to)4798-4810
Number of pages13
JournalJournal of Virology
Volume88
Issue number9
DOIs
StatePublished - Jan 1 2014

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Japanese Encephalitis Virus
Japanese encephalitis virus
MicroRNAs
microRNA
inflammation
neuroglia
Microglia
infection
Infection
interferon-beta
inositols
myo-inositol-1 (or 4)-monophosphatase
Interferon-beta
mice
3' Untranslated Regions
Adaptive Immunity
3' untranslated regions
Virus Diseases
Innate Immunity
phosphotransferases (kinases)

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

MicroRNA 155 regulates Japanese encephalitis virus-induced inflammatory response by targeting Src homology 2-containing inositol phosphatase 1. / Thounaojam, Menaka; Kundu, Kiran; Kaushik, Deepak Kumar; Swaroop, Shalini; Mahadevan, Anita; Shankar, Susarla Krishna; Basu, Anirban.

In: Journal of Virology, Vol. 88, No. 9, 01.01.2014, p. 4798-4810.

Research output: Contribution to journalArticle

Thounaojam, Menaka ; Kundu, Kiran ; Kaushik, Deepak Kumar ; Swaroop, Shalini ; Mahadevan, Anita ; Shankar, Susarla Krishna ; Basu, Anirban. / MicroRNA 155 regulates Japanese encephalitis virus-induced inflammatory response by targeting Src homology 2-containing inositol phosphatase 1. In: Journal of Virology. 2014 ; Vol. 88, No. 9. pp. 4798-4810.
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