MicroRNA-489 induction by hypoxia-inducible factor-1 protects against ischemic kidney injury

Qingqing Wei, Yong Liu, Pengyuan Liu, Jielu Hao, Mingyu Liang, Qing Sheng Mi, Jian Kang Chen, Zheng Dong

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

MicroRNAs have been implicated in ischemic AKI. However, the specific microRNA species that regulates ischemic kidney injury remains unidentified. Our previous microarray analysis revealed microRNA-489 induction in kidneys ofmice subjected to renal ischemia-reperfusion. In this study,we verified the induction of microRNA-489 during ischemic AKI in mice and further examined the underlying mechanisms. Hypoxia-inducible factor-1a deficiency associated with diminished microRNA-489 induction in cultured rat proximal tubular cells subjectedtohypoxia andkidney tissues ofmice after renal ischemia-reperfusioninjury.Moreover, genomic analysis revealed that microRNA-489 is intronic in the calcitonin receptor gene, and chromatin immunoprecipitation assays showed increased binding of hypoxia-inducible factor-1 to a specific site in the calcitonin receptor gene promoter after hypoxia. Inhibition of microRNA-489 increased apoptosis in renal tubular cells after ATP depletion injury in vitro, whereas microRNA-489 mimics mediated protection. Inmice, inhibition of microRNA-489 enhanced tubular cell death and ischemic AKI without significantly affecting tubular cell proliferation. Deep sequencing identified 417 mRNAs that were recruited to the RNA-induced silencing complex by microRNA-489. Of the identified mRNAs, 127 contain microRNA-489 targeting sites, and of those, 18 are involved in the cellular stress response, including the poly(ADP-ribose) polymerase 1 gene implicated in ischemic kidney injury. Sequence analysis and in vitro studies validated poly(ADP-ribose) polymerase 1 as a microRNA-489 target. Together, these results suggest that microRNA-489 is induced via hypoxia-inducible factor-1 during ischemic AKI to protect kidneys by targeting relevant genes.

Original languageEnglish (US)
Pages (from-to)2784-2796
Number of pages13
JournalJournal of the American Society of Nephrology
Volume27
Issue number9
DOIs
StatePublished - 2016

ASJC Scopus subject areas

  • Nephrology

Fingerprint Dive into the research topics of 'MicroRNA-489 induction by hypoxia-inducible factor-1 protects against ischemic kidney injury'. Together they form a unique fingerprint.

Cite this