MicroRNA miR-7 is preferentially expressed in endocrine cells of the developing and adult human pancreas

Mayrin Correa-Medina, Valia Bravo Egana, Samuel Rosero, Camillo Ricordi, Helena Edlund, Juan Diez, Ricardo L. Pastori

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

MicroRNAs (miRNA) are small non-coding RNAs that inhibit gene expression through binding to complementary messenger RNA sequences. miRNAs have been predicted to target genes important for pancreas development, proper endocrine cell function and metabolism. We previously described that miRNA-7 (miR-7) was the most abundant and differentially expressed islet miRNA, with 200-fold higher expression in mature human islets than in acinar tissue. Here we have analyzed the temporal and spatial expression of miR-7 in human fetal pancreas from 8 to 22 weeks of gestational age (wga). Human fetal (8-22 wga) and adult pancreases were processed for immunohistochemistry, in situ hybridization, and quantitative RT-PCR of miRNA and mRNA. miR-7 was expressed in the human developing pancreas from around 9 wga and reached its maximum expression levels between 14 and 18 wga, coinciding with the exponential increase of the pancreatic endocrine hormones. Throughout development miR-7 expression was preferentially localized to endocrine cells and its expression persisted in the adult pancreas. The present study provides a detailed analysis of the spatiotemporal expression of miR-7 in developing human pancreas. The specific localization of miR-7 expression to fetal and adult endocrine cells indicates a potential role for miR-7 in endocrine cell differentiation and/or function. Future functional studies of a potential role for miR-7 function in islet cell differentiation and physiology are likely to identify novel targets for the treatment of diabetes and will lead to the development of improved protocols for generating insulin-producing cells for cell replacement therapy.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalGene Expression Patterns
Volume9
Issue number4
DOIs
StatePublished - Apr 1 2009

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Endocrine Cells
MicroRNAs
Pancreas
Gestational Age
Cell Differentiation
Pancreatic Hormones
Spatio-Temporal Analysis
Small Untranslated RNA
Cell Physiological Phenomena
Complementary RNA
Messenger RNA
Cell- and Tissue-Based Therapy
Islets of Langerhans
In Situ Hybridization
Immunohistochemistry

Keywords

  • Endocrine cells
  • Glucagon
  • Human fetal pancreas
  • In situ hybridization
  • Insulin
  • MicroRNA
  • Neurogenin3
  • Pancreatic islets
  • miR-7

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

MicroRNA miR-7 is preferentially expressed in endocrine cells of the developing and adult human pancreas. / Correa-Medina, Mayrin; Bravo Egana, Valia; Rosero, Samuel; Ricordi, Camillo; Edlund, Helena; Diez, Juan; Pastori, Ricardo L.

In: Gene Expression Patterns, Vol. 9, No. 4, 01.04.2009, p. 193-199.

Research output: Contribution to journalArticle

Correa-Medina, Mayrin ; Bravo Egana, Valia ; Rosero, Samuel ; Ricordi, Camillo ; Edlund, Helena ; Diez, Juan ; Pastori, Ricardo L. / MicroRNA miR-7 is preferentially expressed in endocrine cells of the developing and adult human pancreas. In: Gene Expression Patterns. 2009 ; Vol. 9, No. 4. pp. 193-199.
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