MicroRNAs-141 and 200a regulate the SVCT2 transporter in bone marrow stromal cells

Rajnikumar Sangani, Sudharsan Periyasamy-Thandavan, Ravindra Bharat Kolhe, Maryka H. Bhattacharyya, Norman Chutkan, David M Hunter, Carlos M Isales, Mark W Hamrick, William D. Hill, Sadanand T Fulzele

Research output: Contribution to journalArticle

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Abstract

Vitamin C is a micro-nutrient which plays an important role in bone marrow stromal cell (BMSCs) differentiation to osteogenesis. This vitamin is transported into the BMSCs through the sodium dependent vitamin C transporter 2 (SVCT2). We previously reported that knockdown of the SVCT2 transporter decreases osteogenic differentiation. However, our understanding of the post-transcriptional regulatory mechanism of the SVCT2 transporter remains poor. MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate the messenger RNAs of protein-coding genes. In this study, we aimed to investigate the impact of miR-141 and miR-200a on SVCT2 expression. We found that mouse BMSCs expressed miR-141 and miR-200a and repressed SVCT2 expression at the functional level by targeting the 3'-untranslated region of mRNA. We also found that miR-141 and miR-200a decreased osteogenic differentiation. Furthermore, miRNA inhibitors increased SVCT2 and osteogenic gene expression in BMSCs. Taken together, these results indicate that both miRNAs are novel regulators of the SVCT2 transporter and play an important role in the osteogenic differentiation of BMSCs.

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume410
DOIs
StatePublished - Jul 5 2015

Fingerprint

Sodium-Coupled Vitamin C Transporters
MicroRNAs
Mesenchymal Stromal Cells
Bone
Small Untranslated RNA
Messenger RNA
3' Untranslated Regions
Osteogenesis
Gene expression
Vitamins
Nutrients
Ascorbic Acid
Cell Differentiation
Genes
Gene Expression
Food

Keywords

  • BMSCs
  • miRNA-141
  • miRNA-200a
  • Osteogenic differentiation
  • SVCT2
  • Vitamin C transporter

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

MicroRNAs-141 and 200a regulate the SVCT2 transporter in bone marrow stromal cells. / Sangani, Rajnikumar; Periyasamy-Thandavan, Sudharsan; Kolhe, Ravindra Bharat; Bhattacharyya, Maryka H.; Chutkan, Norman; Hunter, David M; Isales, Carlos M; Hamrick, Mark W; Hill, William D.; Fulzele, Sadanand T.

In: Molecular and Cellular Endocrinology, Vol. 410, 05.07.2015, p. 19-26.

Research output: Contribution to journalArticle

Sangani, Rajnikumar ; Periyasamy-Thandavan, Sudharsan ; Kolhe, Ravindra Bharat ; Bhattacharyya, Maryka H. ; Chutkan, Norman ; Hunter, David M ; Isales, Carlos M ; Hamrick, Mark W ; Hill, William D. ; Fulzele, Sadanand T. / MicroRNAs-141 and 200a regulate the SVCT2 transporter in bone marrow stromal cells. In: Molecular and Cellular Endocrinology. 2015 ; Vol. 410. pp. 19-26.
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