MicroRNAs in islet immunobiology and transplantation

Antonello Pileggi, Dagmar Klein, Carmen Fotino, Valia Bravo-Egaña, Samuel Rosero, Marco Doni, Michele Podetta, Camillo Ricordi, R. Damaris Molano, Ricardo L. Pastori

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The ultimate goal of diabetes therapy is the restoration of physiologic metabolic control. For type 1 diabetes, research efforts are focused on the prevention or early intervention to halt the autoimmune process and preserve β cell function. Replacement of pancreatic β cells via islet transplantation reestablishes physiologic β cell function in patients with diabetes. Emerging research shows that microRNAs (miRNAs), noncoding small RNA molecules produced by a newly discovered class of genes, negatively regulate gene expression. MiRNAs recognize and bind to partially complementary sequences of target messenger RNA (mRNA), regulating mRNA translation and affecting gene expression. Correlation between miRNA signatures and genome-wide RNA expression allows identification of multiple miRNA-mRNA pairs in biological processes. Because miRNAs target functionally related genes, they represent an exciting and indispensable approach for biomarkers and drug discovery. We are studying the role of miRNA in the context of islet immunobiology. Our research aims at understanding the mechanisms underlying pancreatic β cell loss and developing clinically relevant approaches for preservation and restoration of β cell function to treat insulin-dependent diabetes. Herein, we discuss some of our recent efforts related to the study of miRNA in islet inflammation and islet engraftment. Our working hypothesis is that modulation of the expression of specific microRNAs in the transplant microenvironment will be of assistance in enhancing islet engraftment and promoting long-term function.

Original languageEnglish (US)
Pages (from-to)185-196
Number of pages12
JournalImmunologic Research
Volume57
Issue number1-3
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

Keywords

  • Autoimmunity
  • Bioengineering
  • Bioinformatics
  • Biomarker
  • Cell transplantation
  • Cellular therapies
  • Diabetes
  • Diabetes mellitus
  • Engraftment
  • Implantable device
  • Inflammation
  • Innate immunity
  • Islet transplantation
  • MicroRNA
  • Molecular target
  • Neovascularization
  • Rejection
  • Tissue engineering
  • Transplant microenvironment
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology

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  • Cite this

    Pileggi, A., Klein, D., Fotino, C., Bravo-Egaña, V., Rosero, S., Doni, M., Podetta, M., Ricordi, C., Molano, R. D., & Pastori, R. L. (2013). MicroRNAs in islet immunobiology and transplantation. Immunologic Research, 57(1-3), 185-196. https://doi.org/10.1007/s12026-013-8436-5