Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid

R. N. Weller, K. C.Y. Tay, L. V. Garrett, S. Mai, C. M. Primus, J. L. Gutmann, D. H. Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Aim: To investigate the sealing quality of ProRoot Endo Sealer, a calcium silicate-based sealer and its morphologic characteristics after immersion in a phosphate-containing fluid (PCF). Methodology: Single-rooted canals were filled with gutta-percha and either ProRoot Endo Sealer or two commercially available zinc oxide eugenol (ZOE)-based and epoxy resin-based sealers. The sealers were allowed to set for 6 days and the filled teeth were immersed in PCF for 24 h before fluid leakage evaluation. After initial leakage evaluation at the 7th day, each filled root was restored and reimmersed in PCF for 28 days before the second phase of leakage evaluation at 35 days. Cryofractured specimens of additional teeth filled with the three sealers were examined using scanning electron microscopy after immersion in PCF for the two periods. Results: One-way repeated measures anova and Tukey test revealed significant differences between the ZOE-based sealer at 35 days and the calcium silicate-based sealer at 35 days (P < 0.001), and between the ZOE-based sealer at 7 days and the calcium silicate-based sealer at 35 days (P = 0.001). No difference was found between the epoxy resin-based sealer and the calcium silicate-based sealer after both storage periods. Cryofractured calcium silicate-based sealer specimens demonstrated apatite-like crystalline deposits along the apical and middle thirds of the canal walls via transformation from amorphous calcium phosphate-like precursors. Conclusions: ProRoot Endo Sealer is comparable in sealing quality to the epoxy resin-based sealer and seals better than the ZOE-based sealer after immersion in PCF. The calcium silicate-based sealer also demonstrates ex vivo bioactivity when it comes into contact with phosphate ions.

Original languageEnglish (US)
Pages (from-to)977-986
Number of pages10
JournalInternational Endodontic Journal
Volume41
Issue number11
DOIs
StatePublished - Nov 1 2008

Fingerprint

Gutta-Percha
Dental Pulp Cavity
Immersion
Zinc Oxide
Eugenol
Phosphates
Epoxy Resins
Tooth
Apatites
Electron Scanning Microscopy
calcium silicate
Ions

Keywords

  • Calcium silicate-based sealer
  • Flodec
  • Fluid leakage
  • In vitro bioactivity

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid. / Weller, R. N.; Tay, K. C.Y.; Garrett, L. V.; Mai, S.; Primus, C. M.; Gutmann, J. L.; Pashley, D. H.; Tay, Franklin Chi Meng.

In: International Endodontic Journal, Vol. 41, No. 11, 01.11.2008, p. 977-986.

Research output: Contribution to journalArticle

Weller, R. N. ; Tay, K. C.Y. ; Garrett, L. V. ; Mai, S. ; Primus, C. M. ; Gutmann, J. L. ; Pashley, D. H. ; Tay, Franklin Chi Meng. / Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid. In: International Endodontic Journal. 2008 ; Vol. 41, No. 11. pp. 977-986.
@article{57840901df9f4f7f8d3126efaac29a33,
title = "Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid",
abstract = "Aim: To investigate the sealing quality of ProRoot Endo Sealer, a calcium silicate-based sealer and its morphologic characteristics after immersion in a phosphate-containing fluid (PCF). Methodology: Single-rooted canals were filled with gutta-percha and either ProRoot Endo Sealer or two commercially available zinc oxide eugenol (ZOE)-based and epoxy resin-based sealers. The sealers were allowed to set for 6 days and the filled teeth were immersed in PCF for 24 h before fluid leakage evaluation. After initial leakage evaluation at the 7th day, each filled root was restored and reimmersed in PCF for 28 days before the second phase of leakage evaluation at 35 days. Cryofractured specimens of additional teeth filled with the three sealers were examined using scanning electron microscopy after immersion in PCF for the two periods. Results: One-way repeated measures anova and Tukey test revealed significant differences between the ZOE-based sealer at 35 days and the calcium silicate-based sealer at 35 days (P < 0.001), and between the ZOE-based sealer at 7 days and the calcium silicate-based sealer at 35 days (P = 0.001). No difference was found between the epoxy resin-based sealer and the calcium silicate-based sealer after both storage periods. Cryofractured calcium silicate-based sealer specimens demonstrated apatite-like crystalline deposits along the apical and middle thirds of the canal walls via transformation from amorphous calcium phosphate-like precursors. Conclusions: ProRoot Endo Sealer is comparable in sealing quality to the epoxy resin-based sealer and seals better than the ZOE-based sealer after immersion in PCF. The calcium silicate-based sealer also demonstrates ex vivo bioactivity when it comes into contact with phosphate ions.",
keywords = "Calcium silicate-based sealer, Flodec, Fluid leakage, In vitro bioactivity",
author = "Weller, {R. N.} and Tay, {K. C.Y.} and Garrett, {L. V.} and S. Mai and Primus, {C. M.} and Gutmann, {J. L.} and Pashley, {D. H.} and Tay, {Franklin Chi Meng}",
year = "2008",
month = "11",
day = "1",
doi = "10.1111/j.1365-2591.2008.01462.x",
language = "English (US)",
volume = "41",
pages = "977--986",
journal = "International Endodontic Journal",
issn = "0143-2885",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid

AU - Weller, R. N.

AU - Tay, K. C.Y.

AU - Garrett, L. V.

AU - Mai, S.

AU - Primus, C. M.

AU - Gutmann, J. L.

AU - Pashley, D. H.

AU - Tay, Franklin Chi Meng

PY - 2008/11/1

Y1 - 2008/11/1

N2 - Aim: To investigate the sealing quality of ProRoot Endo Sealer, a calcium silicate-based sealer and its morphologic characteristics after immersion in a phosphate-containing fluid (PCF). Methodology: Single-rooted canals were filled with gutta-percha and either ProRoot Endo Sealer or two commercially available zinc oxide eugenol (ZOE)-based and epoxy resin-based sealers. The sealers were allowed to set for 6 days and the filled teeth were immersed in PCF for 24 h before fluid leakage evaluation. After initial leakage evaluation at the 7th day, each filled root was restored and reimmersed in PCF for 28 days before the second phase of leakage evaluation at 35 days. Cryofractured specimens of additional teeth filled with the three sealers were examined using scanning electron microscopy after immersion in PCF for the two periods. Results: One-way repeated measures anova and Tukey test revealed significant differences between the ZOE-based sealer at 35 days and the calcium silicate-based sealer at 35 days (P < 0.001), and between the ZOE-based sealer at 7 days and the calcium silicate-based sealer at 35 days (P = 0.001). No difference was found between the epoxy resin-based sealer and the calcium silicate-based sealer after both storage periods. Cryofractured calcium silicate-based sealer specimens demonstrated apatite-like crystalline deposits along the apical and middle thirds of the canal walls via transformation from amorphous calcium phosphate-like precursors. Conclusions: ProRoot Endo Sealer is comparable in sealing quality to the epoxy resin-based sealer and seals better than the ZOE-based sealer after immersion in PCF. The calcium silicate-based sealer also demonstrates ex vivo bioactivity when it comes into contact with phosphate ions.

AB - Aim: To investigate the sealing quality of ProRoot Endo Sealer, a calcium silicate-based sealer and its morphologic characteristics after immersion in a phosphate-containing fluid (PCF). Methodology: Single-rooted canals were filled with gutta-percha and either ProRoot Endo Sealer or two commercially available zinc oxide eugenol (ZOE)-based and epoxy resin-based sealers. The sealers were allowed to set for 6 days and the filled teeth were immersed in PCF for 24 h before fluid leakage evaluation. After initial leakage evaluation at the 7th day, each filled root was restored and reimmersed in PCF for 28 days before the second phase of leakage evaluation at 35 days. Cryofractured specimens of additional teeth filled with the three sealers were examined using scanning electron microscopy after immersion in PCF for the two periods. Results: One-way repeated measures anova and Tukey test revealed significant differences between the ZOE-based sealer at 35 days and the calcium silicate-based sealer at 35 days (P < 0.001), and between the ZOE-based sealer at 7 days and the calcium silicate-based sealer at 35 days (P = 0.001). No difference was found between the epoxy resin-based sealer and the calcium silicate-based sealer after both storage periods. Cryofractured calcium silicate-based sealer specimens demonstrated apatite-like crystalline deposits along the apical and middle thirds of the canal walls via transformation from amorphous calcium phosphate-like precursors. Conclusions: ProRoot Endo Sealer is comparable in sealing quality to the epoxy resin-based sealer and seals better than the ZOE-based sealer after immersion in PCF. The calcium silicate-based sealer also demonstrates ex vivo bioactivity when it comes into contact with phosphate ions.

KW - Calcium silicate-based sealer

KW - Flodec

KW - Fluid leakage

KW - In vitro bioactivity

UR - http://www.scopus.com/inward/record.url?scp=53849118475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53849118475&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2591.2008.01462.x

DO - 10.1111/j.1365-2591.2008.01462.x

M3 - Article

C2 - 19133087

AN - SCOPUS:53849118475

VL - 41

SP - 977

EP - 986

JO - International Endodontic Journal

JF - International Endodontic Journal

SN - 0143-2885

IS - 11

ER -