Microtubule turnover in ooplasm biopsy reflects ageing phenomena in the parent oocyte

A. P. Goud, Pravin T. Goud, Michael Peter Diamond, P. Van Oostveldt, M. R. Hughes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Oviductal oocytes retrieved from superovulated B6D2F1 mice at 13.5, 16 and 19 h after human chorionic gonadotrophin (HCG) (groups A, B and C respectively, n = 382) were micromanipulated to obtain 12-20 μm sized ooplasm biopsy fragments. Experiments were divided into three sets. Ooplasmic microtubule dynamics were studied in ooplasm biopsy specimens and parent oocytes (set 1) and ooplasm biopsy specimens (set 2), whilst zona pellucida dissolution time, cortical granule loss and spindle/chromatin morphology using confocal microscopy were also studied in parent oocytes (set 2). Oocytes withstood oocyte biopsy with a high survival rate (98.2%) and the biopsied oocytes underwent successful fertilization and development (set 3). An absolute one-to-one correlation was seen between the oocyte biopsy specimens and the parent oocytes in terms of ooplasmic microtubule dynamics (set 1), and increased ooplasmic microtubule dynamics in oocyte biopsy specimens paralleled ageing phenomena in the parent oocytes (set 2). Zona pellucida dissolution time was significantly lower in parent oocytes from group A versus groups B (P = 0.032), and C (P < 0.001). (Groups A, B, C include minimal, moderate, increased ooplasmic microtubule dynamics in oocyte biopsy specimens respectively.) Oocyte cortical granule loss and spindle/chromatin abnormalities were mainly seen in group C (P < 0.001). Oocyte biopsy can thus be applied to judge age-related changes in the parent oocytes.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalReproductive BioMedicine Online
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Microtubules
Oocytes
Biopsy
Zona Pellucida
Chromatin
Chorionic Gonadotropin
Fertilization
Confocal Microscopy

Keywords

  • Cortical granules
  • ICSI
  • Microtubule dynamics
  • Oocyte
  • Ooplasm biopsy
  • Post-ovulatory ageing
  • Spindle

ASJC Scopus subject areas

  • Reproductive Medicine
  • Developmental Biology

Cite this

Microtubule turnover in ooplasm biopsy reflects ageing phenomena in the parent oocyte. / Goud, A. P.; Goud, Pravin T.; Diamond, Michael Peter; Van Oostveldt, P.; Hughes, M. R.

In: Reproductive BioMedicine Online, Vol. 11, No. 1, 01.01.2005, p. 43-52.

Research output: Contribution to journalArticle

Goud, A. P. ; Goud, Pravin T. ; Diamond, Michael Peter ; Van Oostveldt, P. ; Hughes, M. R. / Microtubule turnover in ooplasm biopsy reflects ageing phenomena in the parent oocyte. In: Reproductive BioMedicine Online. 2005 ; Vol. 11, No. 1. pp. 43-52.
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