Microtubules growth rate alteration in human endothelial cells

Irina B. Alieva, Evgeny Alexandrovich Zemskov, Igor I. Kireev, Boris A Gorshkov, Dean A. Wiseman, Stephen Matthew Black, Alexander Dmitriyevich Verin

Research output: Contribution to journalArticle

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Abstract

To understand how microtubules contribute to the dynamic reorganization of the endothelial cell (EC) cytoskeleton, we established an EC model expressing EB3-GFP, a protein that marks microtubule plus-ends. Using this model, we were able to measure microtubule growth rate at the centrosome region and near the cell periphery of a single human EC and in the EC monolayer. We demonstrate that the majority of microtubules in EC are dynamic, the growth rate of their plus-ends is highest in the internal cytoplasm, in the region of the centrosome. Growth rate of microtubule plus-ends decreases from the cell center toward the periphery. Our data suggest the existing mechanism(s) of local regulation of microtubule plus-ends growth in EC. Microtubule growth rate in the internal cytoplasm of EC in the monolayer is lower than that of single EC suggesting the regulatory effect of cell-cell contacts. Centrosomal microtubule growth rate distribution in single EC indicated the presence of two subpopulations of microtubules with "normal" (similar to those inmonolayer EC) and "fast" (three times asmuch) growth rates. Our results indicate functional interactions between cell-cell contacts and microtubules.

Original languageEnglish (US)
Article number671536
JournalJournal of Biomedicine and Biotechnology
Volume2010
DOIs
StatePublished - May 24 2010

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Endothelial cells
Microtubules
Endothelial Cells
Growth
Centrosome
Monolayers
Cytoplasm
Microtubule Proteins
Cytoskeleton
Cell Communication

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

Alieva, I. B., Zemskov, E. A., Kireev, I. I., Gorshkov, B. A., Wiseman, D. A., Black, S. M., & Verin, A. D. (2010). Microtubules growth rate alteration in human endothelial cells. Journal of Biomedicine and Biotechnology, 2010, [671536]. https://doi.org/10.1155/2010/671536

Microtubules growth rate alteration in human endothelial cells. / Alieva, Irina B.; Zemskov, Evgeny Alexandrovich; Kireev, Igor I.; Gorshkov, Boris A; Wiseman, Dean A.; Black, Stephen Matthew; Verin, Alexander Dmitriyevich.

In: Journal of Biomedicine and Biotechnology, Vol. 2010, 671536, 24.05.2010.

Research output: Contribution to journalArticle

Alieva, IB, Zemskov, EA, Kireev, II, Gorshkov, BA, Wiseman, DA, Black, SM & Verin, AD 2010, 'Microtubules growth rate alteration in human endothelial cells', Journal of Biomedicine and Biotechnology, vol. 2010, 671536. https://doi.org/10.1155/2010/671536
Alieva IB, Zemskov EA, Kireev II, Gorshkov BA, Wiseman DA, Black SM et al. Microtubules growth rate alteration in human endothelial cells. Journal of Biomedicine and Biotechnology. 2010 May 24;2010. 671536. https://doi.org/10.1155/2010/671536
Alieva, Irina B. ; Zemskov, Evgeny Alexandrovich ; Kireev, Igor I. ; Gorshkov, Boris A ; Wiseman, Dean A. ; Black, Stephen Matthew ; Verin, Alexander Dmitriyevich. / Microtubules growth rate alteration in human endothelial cells. In: Journal of Biomedicine and Biotechnology. 2010 ; Vol. 2010.
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