Midcycle increase of prolactin seen in normal women is absent in subjects with unexplained infertility

Marappa G. Subramanian, Kenneth A. Ginsburg, Carole L. Kowalczyk, John F. Randolph, Richard E. Leach, Michael Peter Diamond, David M. Lawson, Kamran S. Moghissi, Charla M. Blacker

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To compare the bioactive and immunoactive PRL in normal and unexplained infertility subjects. Design: Prospective study. Setting: Department of Obstetrics and Gynecology, Wayne State University and The University of Michigan. Patient(s): Twelve normal, fertile women compared with 12 patients with unexplained infertility. Intervention(s): Serum samples were obtained across the menstrual cycle and for each subject, 5 pools were prepared by combining serum aliquots from the early follicular, late follicular, midcycle, and midluteal and late luteal phases of the cycle. Main Outcome Measure(s): Niobium lymphoma cell bioassay and an immunoradiometric assay were used to quantitate PRL. Result(s): A midcycle increase in PRL was seen in controls by both assays and these levels were greater compared with other cycle stages. Comparison of midcycle PRL between groups showed differences only between bioactive PRL (34.2 ± 8.3 versus 19.2 ± 3.4 ng/mL [conversion factor to SI unit, 1.00]). The ratios between bioactive and immunoactive PRL were comparable. Significant correlation between bioactive and immunoactive PRL was seen for both control (r = 0.616) and unexplained infertility (r = 0.660) groups. Conclusion(s): The midcycle elevations of bioactive and immunoactive PRL seen in normal women were absent in women with unexplained infertility. This alteration in PRL dynamics may be a part of subtle differences in the reproductive hormone profile of women with unexplained infertility compared with their fertile counterparts.

Original languageEnglish (US)
Pages (from-to)644-647
Number of pages4
JournalFertility and Sterility
Volume67
Issue number4
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

Prolactin
Infertility
International System of Units
Niobium
Immunoradiometric Assay
Hospital Obstetrics and Gynecology Department
Luteal Phase
Menstrual Cycle
Serum
Gynecology
Biological Assay
Lymphoma
Outcome Assessment (Health Care)
Hormones
Prospective Studies

Keywords

  • bioactive prolactin
  • immunoactive prolactin
  • menstrual cycle
  • Prolactin
  • unexplained infertility

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Subramanian, M. G., Ginsburg, K. A., Kowalczyk, C. L., Randolph, J. F., Leach, R. E., Diamond, M. P., ... Blacker, C. M. (1997). Midcycle increase of prolactin seen in normal women is absent in subjects with unexplained infertility. Fertility and Sterility, 67(4), 644-647. https://doi.org/10.1016/S0015-0282(97)81360-X

Midcycle increase of prolactin seen in normal women is absent in subjects with unexplained infertility. / Subramanian, Marappa G.; Ginsburg, Kenneth A.; Kowalczyk, Carole L.; Randolph, John F.; Leach, Richard E.; Diamond, Michael Peter; Lawson, David M.; Moghissi, Kamran S.; Blacker, Charla M.

In: Fertility and Sterility, Vol. 67, No. 4, 01.01.1997, p. 644-647.

Research output: Contribution to journalArticle

Subramanian, MG, Ginsburg, KA, Kowalczyk, CL, Randolph, JF, Leach, RE, Diamond, MP, Lawson, DM, Moghissi, KS & Blacker, CM 1997, 'Midcycle increase of prolactin seen in normal women is absent in subjects with unexplained infertility', Fertility and Sterility, vol. 67, no. 4, pp. 644-647. https://doi.org/10.1016/S0015-0282(97)81360-X
Subramanian, Marappa G. ; Ginsburg, Kenneth A. ; Kowalczyk, Carole L. ; Randolph, John F. ; Leach, Richard E. ; Diamond, Michael Peter ; Lawson, David M. ; Moghissi, Kamran S. ; Blacker, Charla M. / Midcycle increase of prolactin seen in normal women is absent in subjects with unexplained infertility. In: Fertility and Sterility. 1997 ; Vol. 67, No. 4. pp. 644-647.
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abstract = "Objective: To compare the bioactive and immunoactive PRL in normal and unexplained infertility subjects. Design: Prospective study. Setting: Department of Obstetrics and Gynecology, Wayne State University and The University of Michigan. Patient(s): Twelve normal, fertile women compared with 12 patients with unexplained infertility. Intervention(s): Serum samples were obtained across the menstrual cycle and for each subject, 5 pools were prepared by combining serum aliquots from the early follicular, late follicular, midcycle, and midluteal and late luteal phases of the cycle. Main Outcome Measure(s): Niobium lymphoma cell bioassay and an immunoradiometric assay were used to quantitate PRL. Result(s): A midcycle increase in PRL was seen in controls by both assays and these levels were greater compared with other cycle stages. Comparison of midcycle PRL between groups showed differences only between bioactive PRL (34.2 ± 8.3 versus 19.2 ± 3.4 ng/mL [conversion factor to SI unit, 1.00]). The ratios between bioactive and immunoactive PRL were comparable. Significant correlation between bioactive and immunoactive PRL was seen for both control (r = 0.616) and unexplained infertility (r = 0.660) groups. Conclusion(s): The midcycle elevations of bioactive and immunoactive PRL seen in normal women were absent in women with unexplained infertility. This alteration in PRL dynamics may be a part of subtle differences in the reproductive hormone profile of women with unexplained infertility compared with their fertile counterparts.",
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AU - Ginsburg, Kenneth A.

AU - Kowalczyk, Carole L.

AU - Randolph, John F.

AU - Leach, Richard E.

AU - Diamond, Michael Peter

AU - Lawson, David M.

AU - Moghissi, Kamran S.

AU - Blacker, Charla M.

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N2 - Objective: To compare the bioactive and immunoactive PRL in normal and unexplained infertility subjects. Design: Prospective study. Setting: Department of Obstetrics and Gynecology, Wayne State University and The University of Michigan. Patient(s): Twelve normal, fertile women compared with 12 patients with unexplained infertility. Intervention(s): Serum samples were obtained across the menstrual cycle and for each subject, 5 pools were prepared by combining serum aliquots from the early follicular, late follicular, midcycle, and midluteal and late luteal phases of the cycle. Main Outcome Measure(s): Niobium lymphoma cell bioassay and an immunoradiometric assay were used to quantitate PRL. Result(s): A midcycle increase in PRL was seen in controls by both assays and these levels were greater compared with other cycle stages. Comparison of midcycle PRL between groups showed differences only between bioactive PRL (34.2 ± 8.3 versus 19.2 ± 3.4 ng/mL [conversion factor to SI unit, 1.00]). The ratios between bioactive and immunoactive PRL were comparable. Significant correlation between bioactive and immunoactive PRL was seen for both control (r = 0.616) and unexplained infertility (r = 0.660) groups. Conclusion(s): The midcycle elevations of bioactive and immunoactive PRL seen in normal women were absent in women with unexplained infertility. This alteration in PRL dynamics may be a part of subtle differences in the reproductive hormone profile of women with unexplained infertility compared with their fertile counterparts.

AB - Objective: To compare the bioactive and immunoactive PRL in normal and unexplained infertility subjects. Design: Prospective study. Setting: Department of Obstetrics and Gynecology, Wayne State University and The University of Michigan. Patient(s): Twelve normal, fertile women compared with 12 patients with unexplained infertility. Intervention(s): Serum samples were obtained across the menstrual cycle and for each subject, 5 pools were prepared by combining serum aliquots from the early follicular, late follicular, midcycle, and midluteal and late luteal phases of the cycle. Main Outcome Measure(s): Niobium lymphoma cell bioassay and an immunoradiometric assay were used to quantitate PRL. Result(s): A midcycle increase in PRL was seen in controls by both assays and these levels were greater compared with other cycle stages. Comparison of midcycle PRL between groups showed differences only between bioactive PRL (34.2 ± 8.3 versus 19.2 ± 3.4 ng/mL [conversion factor to SI unit, 1.00]). The ratios between bioactive and immunoactive PRL were comparable. Significant correlation between bioactive and immunoactive PRL was seen for both control (r = 0.616) and unexplained infertility (r = 0.660) groups. Conclusion(s): The midcycle elevations of bioactive and immunoactive PRL seen in normal women were absent in women with unexplained infertility. This alteration in PRL dynamics may be a part of subtle differences in the reproductive hormone profile of women with unexplained infertility compared with their fertile counterparts.

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