Mind bomb-binding partner RanBP9 plays a contributory role in retinal development

Kyeong Won Yoo, Maivannan Thiruvarangan, Yun Mi Jeong, Mi Sun Lee, Sateesh Maddirevula, Myungchull Rhee, Young Ki Bae, Hyung Goo Kim, Cheol Hee Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of ranbp9 in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown of ranbp9 resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation marker huC, elevation of neural proliferation marker her4, and alteration of cell cycle marker p57kip2. Expression of the Müller glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bombbinding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalMolecules and Cells
Volume40
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Bombs
Zebrafish
Retina
Retinal Dysplasia
ran GTP-Binding Protein
Differentiation Antigens
Neurogenesis
Brain
Autistic Disorder
Glutamine
Neuroglia
Embryonic Development
Vertebrates
Cultured Cells
Schizophrenia
Cell Cycle
Carrier Proteins
Down-Regulation
Embryonic Structures
Animal Models

Keywords

  • Mind bomb
  • Notch
  • RanBP9
  • Retinogenesis
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Yoo, K. W., Thiruvarangan, M., Jeong, Y. M., Lee, M. S., Maddirevula, S., Rhee, M., ... Kim, C. H. (2017). Mind bomb-binding partner RanBP9 plays a contributory role in retinal development. Molecules and Cells, 40(4), 271-279. https://doi.org/10.14348/molcells.2017.2308

Mind bomb-binding partner RanBP9 plays a contributory role in retinal development. / Yoo, Kyeong Won; Thiruvarangan, Maivannan; Jeong, Yun Mi; Lee, Mi Sun; Maddirevula, Sateesh; Rhee, Myungchull; Bae, Young Ki; Kim, Hyung Goo; Kim, Cheol Hee.

In: Molecules and Cells, Vol. 40, No. 4, 01.04.2017, p. 271-279.

Research output: Contribution to journalArticle

Yoo, KW, Thiruvarangan, M, Jeong, YM, Lee, MS, Maddirevula, S, Rhee, M, Bae, YK, Kim, HG & Kim, CH 2017, 'Mind bomb-binding partner RanBP9 plays a contributory role in retinal development', Molecules and Cells, vol. 40, no. 4, pp. 271-279. https://doi.org/10.14348/molcells.2017.2308
Yoo KW, Thiruvarangan M, Jeong YM, Lee MS, Maddirevula S, Rhee M et al. Mind bomb-binding partner RanBP9 plays a contributory role in retinal development. Molecules and Cells. 2017 Apr 1;40(4):271-279. https://doi.org/10.14348/molcells.2017.2308
Yoo, Kyeong Won ; Thiruvarangan, Maivannan ; Jeong, Yun Mi ; Lee, Mi Sun ; Maddirevula, Sateesh ; Rhee, Myungchull ; Bae, Young Ki ; Kim, Hyung Goo ; Kim, Cheol Hee. / Mind bomb-binding partner RanBP9 plays a contributory role in retinal development. In: Molecules and Cells. 2017 ; Vol. 40, No. 4. pp. 271-279.
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