Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions

Young Kyung Kim, Li Sha Gu, Thomas E. Bryan, Jong R. Kim, Liang Chen, Yan Liu, James C. Yoon, Lorenzo Breschi, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The complex morphologies of mineralised collagen fibrils are regulated through interactions between the collagen matrix and non-collagenous extracellular proteins. In the present study, polyvinylphosphonic acid, a biomimetic analogue of matrix phosphoproteins, was synthesised and confirmed with FTIR and NMR. Biomimetic mineralisation of reconstituted collagen fibrils devoid of natural non-collagenous proteins was demonstrated with TEM using a Portland cement-containing resin composite and a phosphate-containing fluid in the presence of polyacrylic acid as sequestration, and polyvinylphosphonic acid as templating matrix protein analogues. In the presence of these dual biomimetic analogues in the mineralisation medium, intrafibrillar and extrafibrillar mineralisation via bottom-up nanoparticle assembly based on the non-classical crystallisation pathway could be identified. Conversely, only large mineral spheres with no preferred association with collagen fibrils were observed in the absence of biomimetic analogues in the medium. Mineral phases were evident within the collagen fibrils as early as 4 h after the initially-formed amorphous calcium phosphate nanoprecursors were transformed into apatite nanocrystals. Selected area electron diffraction patterns of highly mineralised collagen fibrils were nearly identical to those of natural bone, with apatite crystallites preferentially aligned along the collagen fibril axes.

Original languageEnglish (US)
Pages (from-to)6618-6627
Number of pages10
JournalBiomaterials
Volume31
Issue number25
DOIs
StatePublished - Sep 1 2010

Fingerprint

carbopol 940
Calcium phosphate
Collagen
Biomimetics
Proteins
Acids
Ions
Apatites
Apatite
Nanoparticles
Minerals
Resin Cements
Composite Resins
Phosphoproteins
Fourier Transform Infrared Spectroscopy
Portland cement
Crystallization
calcium phosphate
hydroxide ion
Crystallites

Keywords

  • Extrafibrillar mineralisation
  • Intrafibrillar mineralisation
  • Matrix protein analogues
  • Reconstituted collagen fibrils
  • Tissue engineering materials

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions. / Kim, Young Kyung; Gu, Li Sha; Bryan, Thomas E.; Kim, Jong R.; Chen, Liang; Liu, Yan; Yoon, James C.; Breschi, Lorenzo; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Biomaterials, Vol. 31, No. 25, 01.09.2010, p. 6618-6627.

Research output: Contribution to journalArticle

Kim, Young Kyung ; Gu, Li Sha ; Bryan, Thomas E. ; Kim, Jong R. ; Chen, Liang ; Liu, Yan ; Yoon, James C. ; Breschi, Lorenzo ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions. In: Biomaterials. 2010 ; Vol. 31, No. 25. pp. 6618-6627.
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