Minimal quantities of FSHβ subunit are released by rat anterior pituitary cells in primary culture

H. Edward Grotjan, Albert S. Berkowitz, Brooks Allen Keel

Research output: Contribution to journalArticle

Abstract

FSHβ in the medium and extracts of cultured rat anterior pituitary cells was quantitated with a heterologous radioimmunoassay utilizing anti-rFSHβ and hFSHß as the standard and tracer. After 4 days in culture, extracts were prepared from washed cells, cells incubated for 6 h or 24 h in fresh medium or cells incubated for 6 h in the presence of 10-7 M GnRH. FSHß/rFSH molar ratios were approximately 0.05, 0.04, 0.21 and 0.35, respectively. The elevation in FSHß/rFSH molar ratios in 24 h basal and GnRH-teated cultures was due in part to an increase in intracellular FSHß. Both unstimulated and GnRH-treated cultures contained non-detectable quantities of FSHß in the medium (< 0.063 ng/100000 cells; FSHß/rFSH molar ratio < 0.024). Thus, it appears that cultured rat anterior pituitary cells contain a small amount of uncombined FSHß but that minimal quantities are released. Furthermore, GnRH may increase intracellular quantities of FSHß but does not induce its release.

Original languageEnglish (US)
Pages (from-to)205-209
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume41
Issue number2-3
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

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Primary Cell Culture
Gonadotropin-Releasing Hormone
Rats
Human Follicle Stimulating Hormone
Radioimmunoassay

Keywords

  • FSH
  • FSHβ
  • pituitary

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Minimal quantities of FSHβ subunit are released by rat anterior pituitary cells in primary culture. / Grotjan, H. Edward; Berkowitz, Albert S.; Keel, Brooks Allen.

In: Molecular and Cellular Endocrinology, Vol. 41, No. 2-3, 01.01.1985, p. 205-209.

Research output: Contribution to journalArticle

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abstract = "FSHβ in the medium and extracts of cultured rat anterior pituitary cells was quantitated with a heterologous radioimmunoassay utilizing anti-rFSHβ and hFSH{\ss} as the standard and tracer. After 4 days in culture, extracts were prepared from washed cells, cells incubated for 6 h or 24 h in fresh medium or cells incubated for 6 h in the presence of 10-7 M GnRH. FSH{\ss}/rFSH molar ratios were approximately 0.05, 0.04, 0.21 and 0.35, respectively. The elevation in FSH{\ss}/rFSH molar ratios in 24 h basal and GnRH-teated cultures was due in part to an increase in intracellular FSH{\ss}. Both unstimulated and GnRH-treated cultures contained non-detectable quantities of FSH{\ss} in the medium (< 0.063 ng/100000 cells; FSH{\ss}/rFSH molar ratio < 0.024). Thus, it appears that cultured rat anterior pituitary cells contain a small amount of uncombined FSH{\ss} but that minimal quantities are released. Furthermore, GnRH may increase intracellular quantities of FSH{\ss} but does not induce its release.",
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