MiR-92a regulates viability and angiogenesis of endothelial cells under oxidative stress

Lan Zhang, Mi Zhou, Gangjian Qin, Neal Lee Weintraub, Yao Liang Tang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Oxidative stress contributes to endothelial cell (EC) dysfunction, which is prevalent in ageing and atherosclerosis. MicroRNAs (miRs) are small, non-coding RNAs that post-transcriptionally regulate gene expression and play a key role in fine-tuning EC functional responses, including apoptosis and angiogenesis. MiR-92a is highly expressed in young endothelial cells in comparison with senescent endothelial cells, which exhibit increased oxidative stress and apoptosis. However, the impact of miR-92a treatment on EC viability and angiogenesis under oxidative stress is unknown. Hydrogen peroxide (H 2O2) was used to induce oxidative stress in human umbilical vein endothelial cells (HUVEC). Pre-miR-92a treatment decreased H 2O2-induced apoptosis of HUVEC as determined by TUNEL assay. Pre-miR-92a treatment enhanced capillary tube formation by HUVEC under oxidative stress, which was blocked by LY294002, an inhibitor of Akt phosphorylation. Interestingly, we also observed that inhibition of miR-92a by anti-miR-92a antisense can also enhance angiogenesis in HUVEC with and without oxidative stress exposure. Our results show that perturbation of miR-92a levels outside of its narrow "homeostatic" range may trigger endothelial cell angiogenesis, suggesting that the role of miR-92a in regulating angiogenesis is controversial and may vary depending on the experimental model and method of regulating miR-92a.

Original languageEnglish (US)
Pages (from-to)952-958
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume446
Issue number4
DOIs
StatePublished - Apr 18 2014

Fingerprint

Oxidative stress
Endothelial cells
Oxidative Stress
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Apoptosis
Small Untranslated RNA
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
In Situ Nick-End Labeling
MicroRNAs
Hydrogen Peroxide
Cell Survival
Atherosclerosis
Theoretical Models
Capillary tubes
Phosphorylation
Gene Expression
Gene expression
Assays
Tuning

Keywords

  • Angiogenesis
  • Apoptosis
  • Oxidative stress
  • microRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

MiR-92a regulates viability and angiogenesis of endothelial cells under oxidative stress. / Zhang, Lan; Zhou, Mi; Qin, Gangjian; Weintraub, Neal Lee; Tang, Yao Liang.

In: Biochemical and Biophysical Research Communications, Vol. 446, No. 4, 18.04.2014, p. 952-958.

Research output: Contribution to journalArticle

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