miRNA miR-17-92 cluster is differentially regulated in the imiqumod-treated skin but is not required for imiqumod-induced psoriasis-like dermatitis in mice

Dinghong Wu, Xinling Bi, Le Qu, Ling Han, Congcong Yin, Jingwen Deng, Zheng Dong, Qing Sheng Mi, Li Zhou

Research output: Contribution to journalLetter

3 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) play very important roles in the control of immune cell and keratinocyte development and function and are implicated in skin inflammatory diseases, including psoriasis. miRNA miR-17-92 was reported to promote the differentiation of Th1 and Th1 cells and to regulate cell proliferation and apoptosis. Here we showed that imiquimod (IMQ) differentially regulates the expression of miR-17-92 cluster in the mouse skin, upregulating miR-17 and miR-19 families and downregulating miR-92. To investigate whether miR-17-92 cluster is functionally involved in the psoriasis, we have generated three mutant mice with specific deletion or overexpression of miR-17-92 cluster in keratinocytes, or with deletion of miR-17-92 cluster in T cells. Interestingly, deletion or overexpression of miR-17-92 cluster in keratinocytes, or deletion of miR-17-92 in T cells did not significantly affect IMQ-induced psoriasis-like dermatitis development in the mutant mice compared with wild-type littermates. Thus, miRNA miR-17-92 cluster may not be a key factor regulating imiqumod-induced psoriasis-like dermatitis.

Original languageEnglish (US)
Pages (from-to)82-84
Number of pages3
JournalExperimental Dermatology
Volume26
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Dermatitis
imiquimod
MicroRNAs
Psoriasis
Skin
T-cells
Keratinocytes
Cell proliferation
T-Lymphocytes
Th1 Cells
Skin Diseases
Apoptosis
Down-Regulation
Cell Proliferation

Keywords

  • T cells
  • imiqumod
  • knockin
  • knockout
  • miRNAs
  • psoriasis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

miRNA miR-17-92 cluster is differentially regulated in the imiqumod-treated skin but is not required for imiqumod-induced psoriasis-like dermatitis in mice. / Wu, Dinghong; Bi, Xinling; Qu, Le; Han, Ling; Yin, Congcong; Deng, Jingwen; Dong, Zheng; Mi, Qing Sheng; Zhou, Li.

In: Experimental Dermatology, Vol. 26, No. 1, 01.01.2017, p. 82-84.

Research output: Contribution to journalLetter

Wu, Dinghong ; Bi, Xinling ; Qu, Le ; Han, Ling ; Yin, Congcong ; Deng, Jingwen ; Dong, Zheng ; Mi, Qing Sheng ; Zhou, Li. / miRNA miR-17-92 cluster is differentially regulated in the imiqumod-treated skin but is not required for imiqumod-induced psoriasis-like dermatitis in mice. In: Experimental Dermatology. 2017 ; Vol. 26, No. 1. pp. 82-84.
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abstract = "MicroRNAs (miRNAs) play very important roles in the control of immune cell and keratinocyte development and function and are implicated in skin inflammatory diseases, including psoriasis. miRNA miR-17-92 was reported to promote the differentiation of Th1 and Th1 cells and to regulate cell proliferation and apoptosis. Here we showed that imiquimod (IMQ) differentially regulates the expression of miR-17-92 cluster in the mouse skin, upregulating miR-17 and miR-19 families and downregulating miR-92. To investigate whether miR-17-92 cluster is functionally involved in the psoriasis, we have generated three mutant mice with specific deletion or overexpression of miR-17-92 cluster in keratinocytes, or with deletion of miR-17-92 cluster in T cells. Interestingly, deletion or overexpression of miR-17-92 cluster in keratinocytes, or deletion of miR-17-92 in T cells did not significantly affect IMQ-induced psoriasis-like dermatitis development in the mutant mice compared with wild-type littermates. Thus, miRNA miR-17-92 cluster may not be a key factor regulating imiqumod-induced psoriasis-like dermatitis.",
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AU - Han, Ling

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AU - Mi, Qing Sheng

AU - Zhou, Li

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