Mitochondrial genetic background in Ghanaian patients with primary open-angle glaucoma

Khaled K. Abu-Amero, Michael A. Hauser, Gamal Mohamed, Yutao Liu, Jason Gibson, Ana M. Gonzalez, Stephen Akafo, R. Rand Allingham

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7 Scopus citations

Abstract

Purpose: Prevalence rates for primary open-angle glaucoma (POAG) are significantly higher in Africans than in European or Asians. It has been reported recently that mitochondrial DNA (mtDNA) lineages of African origin, excluding L2, conferred susceptibility to POAG in Saudi Arabia. This prompted us to test the role of mtDNA haplogroups in the incidence of POAG in the Ghanaian population who has a high frequency of L2 lineages. Methods: DNA was extracted from two independent cohorts of clinically diagnosed POAG patients (n=373) and healthy controls (n=451). All patients and controls were from Accra and Tema (the southern region of Ghana). The hypervariable region-I (HVS-I) and coding regions comprising mtDNA haplogroup diagnostic polymorphisms were polymerase chain reaction (PCR) amplified and sequenced in all patients and controls and the haplotypes obtained were assorted into haplogroups and their frequencies compared between cohorts. Results: No statistically significant differences were found in mtDNA haplogroup frequencies between POAG patients and matched controls in this cohort for the various mtDNA haplogroups tested. Conclusions: In this Ghanaian cohort, mtDNA haplogroups do not seem to confer susceptibility to POAG.

Original languageEnglish (US)
Pages (from-to)1955-1959
Number of pages5
JournalMolecular Vision
Volume18
Publication statusPublished - Jul 18 2012
Externally publishedYes

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ASJC Scopus subject areas

  • Ophthalmology

Cite this

Abu-Amero, K. K., Hauser, M. A., Mohamed, G., Liu, Y., Gibson, J., Gonzalez, A. M., ... Rand Allingham, R. (2012). Mitochondrial genetic background in Ghanaian patients with primary open-angle glaucoma. Molecular Vision, 18, 1955-1959.