Mitochondrial nucleoids undergo remodeling in response to metabolic cues

Martin Kucej, Blanka Kucejova, Ramiah Subramanian, Xin Jie Chen, Ronald A. Butow

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Mitochondrial DNA is organized as a nucleoprotein complex called the nucleoid. Its major protein components have been identified in different organisms, but it is yet unknown whether nucleoids undergo any form of remodeling. Using an in organello ChIP-on-chip assay, we demonstrate that the DNA-bending protein Abf2 binds to most of the mitochondrial genome with a preference for GC-rich gene sequences. Thus, Abf2 is a bona fide mitochondrial DNA-packaging protein in vivo. Nucleoids form a more open structure under respiring growth conditions in which the ratio of Abf2 to mitochondrial DNA is decreased. Bifunctional nucleoid proteins Hsp60 and Ilv5 are recruited to nucleoids during glucose repression and amino-acid starvation, respectively. Thus, mitochondrial nucleoids in yeast are dynamic structures that are remodeled in response to metabolic cues. A mutant form of Hsp60 that causes mtDNA instability has altered submitochondrial localization, which suggests that nucleoid remodeling is essential for the maintenance of mitochondrial genome.

Original languageEnglish (US)
Pages (from-to)1861-1868
Number of pages8
JournalJournal of Cell Science
Volume121
Issue number11
DOIs
StatePublished - Jun 1 2008

Fingerprint

Mitochondrial DNA
Cues
Mitochondrial Genome
GC Rich Sequence
DNA Packaging
Chaperonin 60
Proteins
Nucleoproteins
Starvation
Yeasts
Maintenance
Amino Acids
Glucose
DNA
Growth
Genes

Keywords

  • Abf2
  • Hsp60
  • Ilv5
  • Mitochondrial DNA
  • Mitochondrial nucleoid

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kucej, M., Kucejova, B., Subramanian, R., Chen, X. J., & Butow, R. A. (2008). Mitochondrial nucleoids undergo remodeling in response to metabolic cues. Journal of Cell Science, 121(11), 1861-1868. https://doi.org/10.1242/jcs.028605

Mitochondrial nucleoids undergo remodeling in response to metabolic cues. / Kucej, Martin; Kucejova, Blanka; Subramanian, Ramiah; Chen, Xin Jie; Butow, Ronald A.

In: Journal of Cell Science, Vol. 121, No. 11, 01.06.2008, p. 1861-1868.

Research output: Contribution to journalArticle

Kucej, M, Kucejova, B, Subramanian, R, Chen, XJ & Butow, RA 2008, 'Mitochondrial nucleoids undergo remodeling in response to metabolic cues', Journal of Cell Science, vol. 121, no. 11, pp. 1861-1868. https://doi.org/10.1242/jcs.028605
Kucej M, Kucejova B, Subramanian R, Chen XJ, Butow RA. Mitochondrial nucleoids undergo remodeling in response to metabolic cues. Journal of Cell Science. 2008 Jun 1;121(11):1861-1868. https://doi.org/10.1242/jcs.028605
Kucej, Martin ; Kucejova, Blanka ; Subramanian, Ramiah ; Chen, Xin Jie ; Butow, Ronald A. / Mitochondrial nucleoids undergo remodeling in response to metabolic cues. In: Journal of Cell Science. 2008 ; Vol. 121, No. 11. pp. 1861-1868.
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