MK2 SUMOylation regulates actin filament remodeling and subsequent migration in endothelial cells by inhibiting MK2 kinase and HSP27 phosphorylation

Eugene Chang, Kyung Sun Heo, Chang Hoon Woo, Hakjoo Lee, Nhat Tu Le, Tamlyn N. Thomas, Keigi Fujiwara, Jun Ichi Abe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Actin filament remodeling regulates several endothelial cell (EC) processes such as contraction, migration, adhesion, and shape determination. Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2)-mediated phosphorylation of heat-shock protein 27 kDa (HSP27) promotes actin filament remodeling, but little is known about the regulation of this event in ECs.We found that tumor necrosis factor-α (TNF-α) SUMOylated MK2 at lysine (K)-339 affected EC actin filament organization and migration. Loss of the MK2 SUMOylation site (MK2-K339R) increased MK2 kinase activity and prolonged HSP27 phosphorylation, enhancing its effects on actin filament-dependent events. Both TNF-α-mediated EC elongation and steady laminar shear stress-mediated EC alignment were increased by MK2-K339R. Moreover, kinase-dead dominant-negative MK2 (DN-MK2) inhibited these effects. Cell migration is a dynamic process regulated by actin filament remodeling. Both wild-type MK2 (WT-MK2) and DN-MK2 significantly enhanced TNF-mediated inhibition of EC migration, and MK2-K339R further augmented this effect. Interestingly, the p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 reversed this effect by MK2-K339R, which strongly suggests that both excessive and insufficient levels of actin filament remodeling canblockECmigration.Ourstudyshows that MK2 SUMOylation is a new mechanism for regulating actin filament dynamics in ECs.

Original languageEnglish (US)
Pages (from-to)2527-2537
Number of pages11
JournalBlood
Volume117
Issue number8
DOIs
StatePublished - Feb 24 2011
Externally publishedYes

Fingerprint

Sumoylation
Phosphorylation
Endothelial cells
Actin Cytoskeleton
Actins
Endothelial Cells
HSP27 Heat-Shock Proteins
Tumor Necrosis Factor-alpha
Cell Movement
Phosphotransferases
rho-Associated Kinases
MAP-kinase-activated kinase 2
Mitogen-Activated Protein Kinases
Protein Kinases
Lysine
Shear stress
Elongation
Adhesion

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

MK2 SUMOylation regulates actin filament remodeling and subsequent migration in endothelial cells by inhibiting MK2 kinase and HSP27 phosphorylation. / Chang, Eugene; Heo, Kyung Sun; Woo, Chang Hoon; Lee, Hakjoo; Le, Nhat Tu; Thomas, Tamlyn N.; Fujiwara, Keigi; Abe, Jun Ichi.

In: Blood, Vol. 117, No. 8, 24.02.2011, p. 2527-2537.

Research output: Contribution to journalArticle

Chang, Eugene ; Heo, Kyung Sun ; Woo, Chang Hoon ; Lee, Hakjoo ; Le, Nhat Tu ; Thomas, Tamlyn N. ; Fujiwara, Keigi ; Abe, Jun Ichi. / MK2 SUMOylation regulates actin filament remodeling and subsequent migration in endothelial cells by inhibiting MK2 kinase and HSP27 phosphorylation. In: Blood. 2011 ; Vol. 117, No. 8. pp. 2527-2537.
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