MMP-9/TIMP-1 imbalance induced in human dendritic cells by Porphyromonas gingivalis

Ravi Jotwani, Sridhar V.K. Eswaran, Surinder Moonga, Christopher W Cutler

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Matrix metalloproteinase-9 (MMP-9) cleaves collagen, allowing leukocytes to traffic toward the vasculature and the lymphatics. When MMP-9 is unregulated by tissue inhibitor of metalloproteinase-1 (TIMP-1), this can lead to tissue destruction. Dendritic cells (DCs) infiltrate the oral mucosa increasingly in chronic periodontitis, characterized by infection with several pathogens including Porphyromonas gingivalis. In this study, human monocyte-derived DCs were pulsed with different doses of lipopolysaccharide of P. gingivalis 381 and of Escherichia coli type strain 25922, as well as whole live isogenic fimbriae-deficient mutant strains of P. gingivalis 381. Levels of induction of MMP-9 and TIMP-1, as well as interleukin-10 (IL-10), which reportedly inhibits MMP-9 induction, were measured by several approaches. Our results reveal that lipopolysaccharide of P. gingivalis, compared with lipopolysaccharide from E. coli type strain 25922, is a relatively potent inducer of MMP-9, but a weak inducer of TIMP-1, contributing to a high MMP-9/TIMP-1 ratio. Whole live P. gingivalis strain 381, major fimbriae mutant DPG-3 and double mutant MFB were potent inducers of MMP-9, but minor fimbriae mutant MFI was not. MMP-9 induction was inversely proportional to IL-10 induction. These results suggest that lipopolysaccharide and the minor and the major fimbriae of P. gingivalis may play distinct roles in induction by DCs of MMP-9, a potent mediator of local tissue destruction and leukocyte trafficking.

Original languageEnglish (US)
Pages (from-to)314-321
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume58
Issue number3
DOIs
StatePublished - Apr 1 2010

Fingerprint

Porphyromonas gingivalis
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase 9
Dendritic Cells
Lipopolysaccharides
Interleukin-10
Leukocytes
Escherichia coli
Chronic Periodontitis
Matrix Metalloproteinase Inhibitors
Mouth Mucosa
Monocytes
Collagen

Keywords

  • Dendritic cells
  • Lipopolysaccharide
  • MMP-9
  • Periodontitis
  • Porphyromonas gingivalis
  • TIMP-1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

MMP-9/TIMP-1 imbalance induced in human dendritic cells by Porphyromonas gingivalis. / Jotwani, Ravi; Eswaran, Sridhar V.K.; Moonga, Surinder; Cutler, Christopher W.

In: FEMS Immunology and Medical Microbiology, Vol. 58, No. 3, 01.04.2010, p. 314-321.

Research output: Contribution to journalArticle

Jotwani, Ravi ; Eswaran, Sridhar V.K. ; Moonga, Surinder ; Cutler, Christopher W. / MMP-9/TIMP-1 imbalance induced in human dendritic cells by Porphyromonas gingivalis. In: FEMS Immunology and Medical Microbiology. 2010 ; Vol. 58, No. 3. pp. 314-321.
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