Moderating Effect of Low Doses of Ethanol on Reoxygenation Injury in the Anoxic Myocardium

H. Kobayashi, Muhammad Ashraf, P. M. Rahamathulla, M. Minami

Research output: Contribution to journalArticle

Abstract

We have investigated the action of ethanol on the reoxygenation injury in isolated rat hearts. Perfusion of the anoxic Krebs-Henseleit medium for 40 minutes followed by 30 minutes of perfusion with aerobic medium produced considerable myocardial cell injury. Incorporation of ethanol (21.7 mMol), in both anoxic and aerobic perfusion media resulted in a marked reduction of cell injury and inhibition of creative kinase. Contraction band necrosis was reduced to 0.25 as compared to 1.14 per field in the non-treated hearts. The tissue Ca++ was decreased to 8.2 as compared to 12.12 µmoll/g/dry weight in the non-treated hearts and tissue ATP was increased by 50% in the treated tissue (9.33 µmoll/g/dry wt) as compared to the non-treated anoxic tissue (S.S µmol). Thus, ethanol appears to lessen myofibrilar contraction bands and preserve plasma membrane integrity during anoxia and reoxygenation. This suggests a scavenging role of free radicals which include hydroxy radicals or closely related species, in the pathogenesis of anoxic cell injury and beneficial effect of ethanol in low doses on the post anoxic reoxygenation injury.

Original languageEnglish (US)
Pages (from-to)676-684
Number of pages9
JournalPathology Research and Practice
Volume182
Issue number5
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Keywords

  • Calcium ATP
  • Creative kinase
  • Ethanol
  • Reoxygenation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

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