Modulation by gangliosides of the lamellar-inverted micelle (hexagonal II) phase transition in mixtures containing phosphatidylethanolamine and dioleoylglycerol

M. A. Perillo, N. J. Scarsdale, R. K. Yu, B. Maggio

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

We studied the effect of gangliosides GD1a and GM1 on the lamellar-to- hexagonal II phase transition of mixtures of dioleoylphosphatidylethanolamine/dioleoylphosphatidylcholine, 3:1, and of transphosphatidylated phosphatidylethanolamine with dioleoylglycerol by high- sensitivity differential scanning calorimetry, 31P-NMR, and pyrene fluorescence of a phosphatidylcholine probe. Gangliosides had a dual effect. Below 1 mol% ganglioside the hexagonal II phase transition was affected but still occurred at lower temperature than in the absence of gangliosides. The presence of between 1 and 2 mol % gangliosides increased the temperature for formation of the hexagonal II phase and progressively decreased its cooperativity. Above 3 mol % gangliosides totally inhibited the formation of both the temperature-induced and composition-induced hexagonal phase, probably by opposing the geometric distortions necessary for the inverted micellar structures.

Original languageEnglish (US)
Pages (from-to)10019-10023
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number21
DOIs
StatePublished - Oct 11 1994
Externally publishedYes

Keywords

  • P-NMR
  • calorimetry
  • pyrene fluorescence

ASJC Scopus subject areas

  • General

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