Modulation by gangliosides of the lamellar-inverted micelle (hexagonal II) phase transition in mixtures containing phosphatidylethanolamine and dioleoylglycerol

M. A. Perillo, N. J. Scarsdale, Robert K Yu, B. Maggio

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45 Citations (Scopus)

Abstract

We studied the effect of gangliosides GD1a and GM1 on the lamellar-to- hexagonal II phase transition of mixtures of dioleoylphosphatidylethanolamine/dioleoylphosphatidylcholine, 3:1, and of transphosphatidylated phosphatidylethanolamine with dioleoylglycerol by high- sensitivity differential scanning calorimetry, 31P-NMR, and pyrene fluorescence of a phosphatidylcholine probe. Gangliosides had a dual effect. Below 1 mol% ganglioside the hexagonal II phase transition was affected but still occurred at lower temperature than in the absence of gangliosides. The presence of between 1 and 2 mol % gangliosides increased the temperature for formation of the hexagonal II phase and progressively decreased its cooperativity. Above 3 mol % gangliosides totally inhibited the formation of both the temperature-induced and composition-induced hexagonal phase, probably by opposing the geometric distortions necessary for the inverted micellar structures.

Original languageEnglish (US)
Pages (from-to)10019-10023
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number21
DOIs
StatePublished - Oct 11 1994

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Gangliosides
Phase Transition
Micelles
Temperature
G(M1) Ganglioside
Differential Scanning Calorimetry
Phosphatidylcholines
Fluorescence
diolein
phosphatidylethanolamine

Keywords

  • P-NMR
  • calorimetry
  • pyrene fluorescence

ASJC Scopus subject areas

  • General

Cite this

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title = "Modulation by gangliosides of the lamellar-inverted micelle (hexagonal II) phase transition in mixtures containing phosphatidylethanolamine and dioleoylglycerol",
abstract = "We studied the effect of gangliosides GD1a and GM1 on the lamellar-to- hexagonal II phase transition of mixtures of dioleoylphosphatidylethanolamine/dioleoylphosphatidylcholine, 3:1, and of transphosphatidylated phosphatidylethanolamine with dioleoylglycerol by high- sensitivity differential scanning calorimetry, 31P-NMR, and pyrene fluorescence of a phosphatidylcholine probe. Gangliosides had a dual effect. Below 1 mol{\%} ganglioside the hexagonal II phase transition was affected but still occurred at lower temperature than in the absence of gangliosides. The presence of between 1 and 2 mol {\%} gangliosides increased the temperature for formation of the hexagonal II phase and progressively decreased its cooperativity. Above 3 mol {\%} gangliosides totally inhibited the formation of both the temperature-induced and composition-induced hexagonal phase, probably by opposing the geometric distortions necessary for the inverted micellar structures.",
keywords = "P-NMR, calorimetry, pyrene fluorescence",
author = "Perillo, {M. A.} and Scarsdale, {N. J.} and Yu, {Robert K} and B. Maggio",
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doi = "10.1073/pnas.91.21.10019",
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T1 - Modulation by gangliosides of the lamellar-inverted micelle (hexagonal II) phase transition in mixtures containing phosphatidylethanolamine and dioleoylglycerol

AU - Perillo, M. A.

AU - Scarsdale, N. J.

AU - Yu, Robert K

AU - Maggio, B.

PY - 1994/10/11

Y1 - 1994/10/11

N2 - We studied the effect of gangliosides GD1a and GM1 on the lamellar-to- hexagonal II phase transition of mixtures of dioleoylphosphatidylethanolamine/dioleoylphosphatidylcholine, 3:1, and of transphosphatidylated phosphatidylethanolamine with dioleoylglycerol by high- sensitivity differential scanning calorimetry, 31P-NMR, and pyrene fluorescence of a phosphatidylcholine probe. Gangliosides had a dual effect. Below 1 mol% ganglioside the hexagonal II phase transition was affected but still occurred at lower temperature than in the absence of gangliosides. The presence of between 1 and 2 mol % gangliosides increased the temperature for formation of the hexagonal II phase and progressively decreased its cooperativity. Above 3 mol % gangliosides totally inhibited the formation of both the temperature-induced and composition-induced hexagonal phase, probably by opposing the geometric distortions necessary for the inverted micellar structures.

AB - We studied the effect of gangliosides GD1a and GM1 on the lamellar-to- hexagonal II phase transition of mixtures of dioleoylphosphatidylethanolamine/dioleoylphosphatidylcholine, 3:1, and of transphosphatidylated phosphatidylethanolamine with dioleoylglycerol by high- sensitivity differential scanning calorimetry, 31P-NMR, and pyrene fluorescence of a phosphatidylcholine probe. Gangliosides had a dual effect. Below 1 mol% ganglioside the hexagonal II phase transition was affected but still occurred at lower temperature than in the absence of gangliosides. The presence of between 1 and 2 mol % gangliosides increased the temperature for formation of the hexagonal II phase and progressively decreased its cooperativity. Above 3 mol % gangliosides totally inhibited the formation of both the temperature-induced and composition-induced hexagonal phase, probably by opposing the geometric distortions necessary for the inverted micellar structures.

KW - P-NMR

KW - calorimetry

KW - pyrene fluorescence

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U2 - 10.1073/pnas.91.21.10019

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