Modulation of aromatase activity by growth factors in an androgen sensitive human prostate cancer cell line, LNCaP

Janice L. Block, Norman L. Block, Balakrishna L Lokeshwar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effects of steroids and peptide growth factors on aromatase activity in an androgen sensitive human prostate cancer cell line (LNCaP) were investigated. Factors were selected based on their observed modulation of the enzyme in other tissues. Incubation with epidermal growth factor and transforming growth factor-1 decreased aromatase activity in LNCaP cells by 25-40%. Insulin like growth factor-1, dexamethasone, dibutyryl cAMP and phorbol 12-myristate 13-acetate, all of which are modulators of aromatase in other tissues, had no significant effect on aromatase activity in LNCaP cells. In addition, the cAMP-dependent protein kinase and protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2 methylpiperazine (H-7) had no effect on the enzyme activity. Factors affecting prostatic aromatase may be distinct from those for other known species.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalCancer Letters
Volume102
Issue number1-2
DOIs
StatePublished - Apr 19 1996
Externally publishedYes

Fingerprint

Aromatase
Androgens
Prostatic Neoplasms
Intercellular Signaling Peptides and Proteins
Cell Line
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Protein C Inhibitor
Transforming Growth Factors
Somatomedins
Enzymes
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
Epidermal Growth Factor
Protein Kinase C
Dexamethasone
Acetates
Steroids
Peptides

Keywords

  • Androgen
  • Aromatase
  • Growth factors
  • Prostate cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Modulation of aromatase activity by growth factors in an androgen sensitive human prostate cancer cell line, LNCaP. / Block, Janice L.; Block, Norman L.; Lokeshwar, Balakrishna L.

In: Cancer Letters, Vol. 102, No. 1-2, 19.04.1996, p. 167-172.

Research output: Contribution to journalArticle

@article{f0c4de67b8de498db904022621221b5c,
title = "Modulation of aromatase activity by growth factors in an androgen sensitive human prostate cancer cell line, LNCaP",
abstract = "The effects of steroids and peptide growth factors on aromatase activity in an androgen sensitive human prostate cancer cell line (LNCaP) were investigated. Factors were selected based on their observed modulation of the enzyme in other tissues. Incubation with epidermal growth factor and transforming growth factor-1 decreased aromatase activity in LNCaP cells by 25-40{\%}. Insulin like growth factor-1, dexamethasone, dibutyryl cAMP and phorbol 12-myristate 13-acetate, all of which are modulators of aromatase in other tissues, had no significant effect on aromatase activity in LNCaP cells. In addition, the cAMP-dependent protein kinase and protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2 methylpiperazine (H-7) had no effect on the enzyme activity. Factors affecting prostatic aromatase may be distinct from those for other known species.",
keywords = "Androgen, Aromatase, Growth factors, Prostate cancer",
author = "Block, {Janice L.} and Block, {Norman L.} and Lokeshwar, {Balakrishna L}",
year = "1996",
month = "4",
day = "19",
doi = "10.1016/0304-3835(96)04176-6",
language = "English (US)",
volume = "102",
pages = "167--172",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Modulation of aromatase activity by growth factors in an androgen sensitive human prostate cancer cell line, LNCaP

AU - Block, Janice L.

AU - Block, Norman L.

AU - Lokeshwar, Balakrishna L

PY - 1996/4/19

Y1 - 1996/4/19

N2 - The effects of steroids and peptide growth factors on aromatase activity in an androgen sensitive human prostate cancer cell line (LNCaP) were investigated. Factors were selected based on their observed modulation of the enzyme in other tissues. Incubation with epidermal growth factor and transforming growth factor-1 decreased aromatase activity in LNCaP cells by 25-40%. Insulin like growth factor-1, dexamethasone, dibutyryl cAMP and phorbol 12-myristate 13-acetate, all of which are modulators of aromatase in other tissues, had no significant effect on aromatase activity in LNCaP cells. In addition, the cAMP-dependent protein kinase and protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2 methylpiperazine (H-7) had no effect on the enzyme activity. Factors affecting prostatic aromatase may be distinct from those for other known species.

AB - The effects of steroids and peptide growth factors on aromatase activity in an androgen sensitive human prostate cancer cell line (LNCaP) were investigated. Factors were selected based on their observed modulation of the enzyme in other tissues. Incubation with epidermal growth factor and transforming growth factor-1 decreased aromatase activity in LNCaP cells by 25-40%. Insulin like growth factor-1, dexamethasone, dibutyryl cAMP and phorbol 12-myristate 13-acetate, all of which are modulators of aromatase in other tissues, had no significant effect on aromatase activity in LNCaP cells. In addition, the cAMP-dependent protein kinase and protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2 methylpiperazine (H-7) had no effect on the enzyme activity. Factors affecting prostatic aromatase may be distinct from those for other known species.

KW - Androgen

KW - Aromatase

KW - Growth factors

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=0029967872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029967872&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(96)04176-6

DO - 10.1016/0304-3835(96)04176-6

M3 - Article

VL - 102

SP - 167

EP - 172

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1-2

ER -