Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates

Xuetong Shen, Hua Xiao, Ryan Ranallo, Wei-Hua Wu, Carl Wu

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    Abstract

    Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin- remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakis-phosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.

    Original languageEnglish (US)
    Pages (from-to)112-114
    Number of pages3
    JournalScience
    Volume299
    Issue number5603
    DOIs
    Publication statusPublished - Jan 3 2003

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