TY - JOUR
T1 - Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates
AU - Shen, Xuetong
AU - Xiao, Hua
AU - Ranallo, Ryan
AU - Wu, Wei Hua
AU - Wu, Carl
PY - 2003/1/3
Y1 - 2003/1/3
N2 - Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin- remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakis-phosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.
AB - Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin- remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakis-phosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.
UR - http://www.scopus.com/inward/record.url?scp=0037414839&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037414839&partnerID=8YFLogxK
U2 - 10.1126/science.1078068
DO - 10.1126/science.1078068
M3 - Article
C2 - 12434013
AN - SCOPUS:0037414839
SN - 0036-8075
VL - 299
SP - 112
EP - 114
JO - Science
JF - Science
IS - 5603
ER -