Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates

Xuetong Shen, Hua Xiao, Ryan Ranallo, Wei-Hua Wu, Carl Wu

    Research output: Contribution to journalArticle

    266 Citations (Scopus)

    Abstract

    Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin- remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakis-phosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.

    Original languageEnglish (US)
    Pages (from-to)112-114
    Number of pages3
    JournalScience
    Volume299
    Issue number5603
    DOIs
    StatePublished - Jan 3 2003

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    Polyphosphates
    Chromatin Assembly and Disassembly
    Inositol
    Nucleosomes
    Adenosine Triphosphate
    Gene Expression
    Phytic Acid
    Inositol Phosphates
    Eukaryota
    Yeasts
    Mutation
    Genes

    ASJC Scopus subject areas

    • General

    Cite this

    Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates. / Shen, Xuetong; Xiao, Hua; Ranallo, Ryan; Wu, Wei-Hua; Wu, Carl.

    In: Science, Vol. 299, No. 5603, 03.01.2003, p. 112-114.

    Research output: Contribution to journalArticle

    Shen, X, Xiao, H, Ranallo, R, Wu, W-H & Wu, C 2003, 'Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates', Science, vol. 299, no. 5603, pp. 112-114. https://doi.org/10.1126/science.1078068
    Shen, Xuetong ; Xiao, Hua ; Ranallo, Ryan ; Wu, Wei-Hua ; Wu, Carl. / Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates. In: Science. 2003 ; Vol. 299, No. 5603. pp. 112-114.
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