Modulation of miRNAS by vitamin C in human bone marrow stromal cells

Ravindra Bharat Kolhe, Ashis K. Mondal, Chetan Pundkar, Sudharsan Periyasamy Thandavan, Bharati Mendhe, David M Hunter, Carlos M Isales, William D Hill, Mark W Hamrick, Sadanand T Fulzele

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small (18–25 nucleotides), noncoding RNAs that have been identified as potential regulators of bone marrow stromal cell (BMSC) proliferation, differentiation, and musculoskeletal development. Vitamin C is known to play a vital role in such types of biological processes through various different mechanisms by altering mRNA expression. We hypothesized that vitamin C mediates these biological processes partially through miRNA regulation. We performed global miRNA expression analysis on human BMSCs following vitamin C treatment using microarrays containing human precursor and mature miRNA probes. Bioinformatics analyses were performed on differentially expressed miRNAs to identify novel target genes and signaling pathways. Our bioinformatics analysis suggested that the miRNAs may regulate multiple stem cell-specific signaling pathways such as cell adhesion molecules (CAMs), fatty acid biosynthesis and hormone signaling pathways. Furthermore, our analysis predicted novel stem cell proliferation and differentiation gene targets. The findings of the present study demonstrate that vitamin C can have positive effects on BMSCs in part by regulating miRNA expression.

Original languageEnglish (US)
Article number186
JournalNutrients
Volume10
Issue number2
DOIs
StatePublished - Feb 8 2018

Fingerprint

stromal cells
bone marrow cells
MicroRNAs
Mesenchymal Stromal Cells
microRNA
Ascorbic Acid
ascorbic acid
Biological Phenomena
Computational Biology
bioinformatics
stem cells
Cell Differentiation
cell proliferation
Musculoskeletal Development
Stem Cells
Cell Proliferation
Untranslated RNA
Cell Adhesion Molecules
cell adhesion
cell differentiation

Keywords

  • Bone marrow stromal cells
  • Vitamin C
  • miRNA

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Modulation of miRNAS by vitamin C in human bone marrow stromal cells. / Kolhe, Ravindra Bharat; Mondal, Ashis K.; Pundkar, Chetan; Periyasamy Thandavan, Sudharsan; Mendhe, Bharati; Hunter, David M; Isales, Carlos M; Hill, William D; Hamrick, Mark W; Fulzele, Sadanand T.

In: Nutrients, Vol. 10, No. 2, 186, 08.02.2018.

Research output: Contribution to journalArticle

Kolhe RB, Mondal AK, Pundkar C, Periyasamy Thandavan S, Mendhe B, Hunter DM et al. Modulation of miRNAS by vitamin C in human bone marrow stromal cells. Nutrients. 2018 Feb 8;10(2). 186. https://doi.org/10.3390/nu10020186
Kolhe, Ravindra Bharat ; Mondal, Ashis K. ; Pundkar, Chetan ; Periyasamy Thandavan, Sudharsan ; Mendhe, Bharati ; Hunter, David M ; Isales, Carlos M ; Hill, William D ; Hamrick, Mark W ; Fulzele, Sadanand T. / Modulation of miRNAS by vitamin C in human bone marrow stromal cells. In: Nutrients. 2018 ; Vol. 10, No. 2.
@article{8b35fed193dc4155881eec019e51798b,
title = "Modulation of miRNAS by vitamin C in human bone marrow stromal cells",
abstract = "MicroRNAs (miRNAs) are small (18–25 nucleotides), noncoding RNAs that have been identified as potential regulators of bone marrow stromal cell (BMSC) proliferation, differentiation, and musculoskeletal development. Vitamin C is known to play a vital role in such types of biological processes through various different mechanisms by altering mRNA expression. We hypothesized that vitamin C mediates these biological processes partially through miRNA regulation. We performed global miRNA expression analysis on human BMSCs following vitamin C treatment using microarrays containing human precursor and mature miRNA probes. Bioinformatics analyses were performed on differentially expressed miRNAs to identify novel target genes and signaling pathways. Our bioinformatics analysis suggested that the miRNAs may regulate multiple stem cell-specific signaling pathways such as cell adhesion molecules (CAMs), fatty acid biosynthesis and hormone signaling pathways. Furthermore, our analysis predicted novel stem cell proliferation and differentiation gene targets. The findings of the present study demonstrate that vitamin C can have positive effects on BMSCs in part by regulating miRNA expression.",
keywords = "Bone marrow stromal cells, Vitamin C, miRNA",
author = "Kolhe, {Ravindra Bharat} and Mondal, {Ashis K.} and Chetan Pundkar and {Periyasamy Thandavan}, Sudharsan and Bharati Mendhe and Hunter, {David M} and Isales, {Carlos M} and Hill, {William D} and Hamrick, {Mark W} and Fulzele, {Sadanand T}",
year = "2018",
month = "2",
day = "8",
doi = "10.3390/nu10020186",
language = "English (US)",
volume = "10",
journal = "Nutrients",
issn = "2072-6643",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

TY - JOUR

T1 - Modulation of miRNAS by vitamin C in human bone marrow stromal cells

AU - Kolhe, Ravindra Bharat

AU - Mondal, Ashis K.

AU - Pundkar, Chetan

AU - Periyasamy Thandavan, Sudharsan

AU - Mendhe, Bharati

AU - Hunter, David M

AU - Isales, Carlos M

AU - Hill, William D

AU - Hamrick, Mark W

AU - Fulzele, Sadanand T

PY - 2018/2/8

Y1 - 2018/2/8

N2 - MicroRNAs (miRNAs) are small (18–25 nucleotides), noncoding RNAs that have been identified as potential regulators of bone marrow stromal cell (BMSC) proliferation, differentiation, and musculoskeletal development. Vitamin C is known to play a vital role in such types of biological processes through various different mechanisms by altering mRNA expression. We hypothesized that vitamin C mediates these biological processes partially through miRNA regulation. We performed global miRNA expression analysis on human BMSCs following vitamin C treatment using microarrays containing human precursor and mature miRNA probes. Bioinformatics analyses were performed on differentially expressed miRNAs to identify novel target genes and signaling pathways. Our bioinformatics analysis suggested that the miRNAs may regulate multiple stem cell-specific signaling pathways such as cell adhesion molecules (CAMs), fatty acid biosynthesis and hormone signaling pathways. Furthermore, our analysis predicted novel stem cell proliferation and differentiation gene targets. The findings of the present study demonstrate that vitamin C can have positive effects on BMSCs in part by regulating miRNA expression.

AB - MicroRNAs (miRNAs) are small (18–25 nucleotides), noncoding RNAs that have been identified as potential regulators of bone marrow stromal cell (BMSC) proliferation, differentiation, and musculoskeletal development. Vitamin C is known to play a vital role in such types of biological processes through various different mechanisms by altering mRNA expression. We hypothesized that vitamin C mediates these biological processes partially through miRNA regulation. We performed global miRNA expression analysis on human BMSCs following vitamin C treatment using microarrays containing human precursor and mature miRNA probes. Bioinformatics analyses were performed on differentially expressed miRNAs to identify novel target genes and signaling pathways. Our bioinformatics analysis suggested that the miRNAs may regulate multiple stem cell-specific signaling pathways such as cell adhesion molecules (CAMs), fatty acid biosynthesis and hormone signaling pathways. Furthermore, our analysis predicted novel stem cell proliferation and differentiation gene targets. The findings of the present study demonstrate that vitamin C can have positive effects on BMSCs in part by regulating miRNA expression.

KW - Bone marrow stromal cells

KW - Vitamin C

KW - miRNA

UR - http://www.scopus.com/inward/record.url?scp=85041943848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041943848&partnerID=8YFLogxK

U2 - 10.3390/nu10020186

DO - 10.3390/nu10020186

M3 - Article

C2 - 29419776

AN - SCOPUS:85041943848

VL - 10

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 2

M1 - 186

ER -