Modulation of phospholipases a2 and c activities against dilauroylphosphorylcholine in mixed monolayers with semisynthetic derivatives of ganglioside and sphingosine

Marí A. Perillo, Alessandro Guidotti, Erminio Costa, Robert K Yu, Bruno Maggio

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Summary Most of the semisynthetic ganglioside and sphingosine derivatives studied here decreased the rate as well as the extent of hydrolysis of monomolecular layers of dilauroylphosphorylcholine (dIPC) by both phospholipase A2 (PLA2) and C (PLC). For PLA2, the rate of enzymatic activity was inversely correlated (p<0-001) with the duration of the latency period of the enzymatic reaction. The correlation between the rate of activity and the latency period was not statistically significant for PLC. The potency to inhibit PLA2 and PLC was not significantly correlated with the presence of specific chemical groups. Also, the inhibitory effect is not correlated to changes of the substrate intermolecular spacing or surface potential caused by the sphingolipids (SLs). Conversely, for PLA2 the variation of the kinetic parameters of the reaction with the molecular polarization vector of the SLs are statistically significant (p<0-01). The rate of phospholipid degradation by PLA2 decreased, and the lag times tended to become longer, with increasing values of the SLs' polarization vector. The kinetic parameters of the reaction with PLC did not show statistically significant correlation with the polarization vector of the SLs. Our results suggest that the configuration of the electrostatic field across the interface is more important than are formal charges or specific chemical moieties in modulating the activity of PLA2. Inhibition of phospholipase activities of these types by specific SLs or their metabolites may be important as supramolecular regulatory steps at the membrane level of the amplification of lipid-mediated signalling pathways.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalMolecular Membrane Biology
Volume11
Issue number2
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Sphingosine
Gangliosides
Phospholipases A2
Sphingolipids
Type C Phospholipases
Phospholipases
Static Electricity
Phospholipids
Hydrolysis
Lipids
Membranes

Keywords

  • Gangliosides
  • Monolayers
  • Phospholipase C
  • Sphingosine derivatives

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Modulation of phospholipases a2 and c activities against dilauroylphosphorylcholine in mixed monolayers with semisynthetic derivatives of ganglioside and sphingosine. / Perillo, Marí A.; Guidotti, Alessandro; Costa, Erminio; Yu, Robert K; Maggio, Bruno.

In: Molecular Membrane Biology, Vol. 11, No. 2, 01.01.1994, p. 119-126.

Research output: Contribution to journalArticle

@article{c89156fd9bc14322a5cc0953b52ea12c,
title = "Modulation of phospholipases a2 and c activities against dilauroylphosphorylcholine in mixed monolayers with semisynthetic derivatives of ganglioside and sphingosine",
abstract = "Summary Most of the semisynthetic ganglioside and sphingosine derivatives studied here decreased the rate as well as the extent of hydrolysis of monomolecular layers of dilauroylphosphorylcholine (dIPC) by both phospholipase A2 (PLA2) and C (PLC). For PLA2, the rate of enzymatic activity was inversely correlated (p<0-001) with the duration of the latency period of the enzymatic reaction. The correlation between the rate of activity and the latency period was not statistically significant for PLC. The potency to inhibit PLA2 and PLC was not significantly correlated with the presence of specific chemical groups. Also, the inhibitory effect is not correlated to changes of the substrate intermolecular spacing or surface potential caused by the sphingolipids (SLs). Conversely, for PLA2 the variation of the kinetic parameters of the reaction with the molecular polarization vector of the SLs are statistically significant (p<0-01). The rate of phospholipid degradation by PLA2 decreased, and the lag times tended to become longer, with increasing values of the SLs' polarization vector. The kinetic parameters of the reaction with PLC did not show statistically significant correlation with the polarization vector of the SLs. Our results suggest that the configuration of the electrostatic field across the interface is more important than are formal charges or specific chemical moieties in modulating the activity of PLA2. Inhibition of phospholipase activities of these types by specific SLs or their metabolites may be important as supramolecular regulatory steps at the membrane level of the amplification of lipid-mediated signalling pathways.",
keywords = "Gangliosides, Monolayers, Phospholipase C, Sphingosine derivatives",
author = "Perillo, {Mar{\'i} A.} and Alessandro Guidotti and Erminio Costa and Yu, {Robert K} and Bruno Maggio",
year = "1994",
month = "1",
day = "1",
doi = "10.3109/09687689409162229",
language = "English (US)",
volume = "11",
pages = "119--126",
journal = "Molecular Membrane Biology",
issn = "0968-7688",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Modulation of phospholipases a2 and c activities against dilauroylphosphorylcholine in mixed monolayers with semisynthetic derivatives of ganglioside and sphingosine

AU - Perillo, Marí A.

AU - Guidotti, Alessandro

AU - Costa, Erminio

AU - Yu, Robert K

AU - Maggio, Bruno

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Summary Most of the semisynthetic ganglioside and sphingosine derivatives studied here decreased the rate as well as the extent of hydrolysis of monomolecular layers of dilauroylphosphorylcholine (dIPC) by both phospholipase A2 (PLA2) and C (PLC). For PLA2, the rate of enzymatic activity was inversely correlated (p<0-001) with the duration of the latency period of the enzymatic reaction. The correlation between the rate of activity and the latency period was not statistically significant for PLC. The potency to inhibit PLA2 and PLC was not significantly correlated with the presence of specific chemical groups. Also, the inhibitory effect is not correlated to changes of the substrate intermolecular spacing or surface potential caused by the sphingolipids (SLs). Conversely, for PLA2 the variation of the kinetic parameters of the reaction with the molecular polarization vector of the SLs are statistically significant (p<0-01). The rate of phospholipid degradation by PLA2 decreased, and the lag times tended to become longer, with increasing values of the SLs' polarization vector. The kinetic parameters of the reaction with PLC did not show statistically significant correlation with the polarization vector of the SLs. Our results suggest that the configuration of the electrostatic field across the interface is more important than are formal charges or specific chemical moieties in modulating the activity of PLA2. Inhibition of phospholipase activities of these types by specific SLs or their metabolites may be important as supramolecular regulatory steps at the membrane level of the amplification of lipid-mediated signalling pathways.

AB - Summary Most of the semisynthetic ganglioside and sphingosine derivatives studied here decreased the rate as well as the extent of hydrolysis of monomolecular layers of dilauroylphosphorylcholine (dIPC) by both phospholipase A2 (PLA2) and C (PLC). For PLA2, the rate of enzymatic activity was inversely correlated (p<0-001) with the duration of the latency period of the enzymatic reaction. The correlation between the rate of activity and the latency period was not statistically significant for PLC. The potency to inhibit PLA2 and PLC was not significantly correlated with the presence of specific chemical groups. Also, the inhibitory effect is not correlated to changes of the substrate intermolecular spacing or surface potential caused by the sphingolipids (SLs). Conversely, for PLA2 the variation of the kinetic parameters of the reaction with the molecular polarization vector of the SLs are statistically significant (p<0-01). The rate of phospholipid degradation by PLA2 decreased, and the lag times tended to become longer, with increasing values of the SLs' polarization vector. The kinetic parameters of the reaction with PLC did not show statistically significant correlation with the polarization vector of the SLs. Our results suggest that the configuration of the electrostatic field across the interface is more important than are formal charges or specific chemical moieties in modulating the activity of PLA2. Inhibition of phospholipase activities of these types by specific SLs or their metabolites may be important as supramolecular regulatory steps at the membrane level of the amplification of lipid-mediated signalling pathways.

KW - Gangliosides

KW - Monolayers

KW - Phospholipase C

KW - Sphingosine derivatives

UR - http://www.scopus.com/inward/record.url?scp=0028411174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028411174&partnerID=8YFLogxK

U2 - 10.3109/09687689409162229

DO - 10.3109/09687689409162229

M3 - Article

C2 - 7920864

AN - SCOPUS:0028411174

VL - 11

SP - 119

EP - 126

JO - Molecular Membrane Biology

JF - Molecular Membrane Biology

SN - 0968-7688

IS - 2

ER -