Modulation of restraint stress induced oxidative changes in rats by antioxidant vitamins

S. M.Kashif R. Zaidi, Tariq M. Al-Qirim, MD Nasrul Hoda, Naheed Banu

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

In the present study we examined immobilization stress-induced antioxidant defense changes in rat plasma and also observed the antioxidant effects of pre and post vitamins A, E and C administration (15 mg/Kg of body weight) individually and in combination (vit E + C) on these alterations. Following immobilization stress the circulating activities of superoxide dismutase, catalase and glutathione-S-transferase were decreased, while the level of thiobarbituric acid reactive substances (TBARS) was increased as compared to non-stressed control rats. Post treatment with individual vitamins A, E and C (after exposure to stress) resulted in a less marked alteration of plasma TBARS levels and activities of SOD, GST and catalase as compared to pre vitamin stress or stress alone treatments. Both pre and post vitamin treatments were effective in preventing stress induced derangement of free radical metabolism with a relative dominance by latter. The combined treatment with vitamin E and C did not show any additive antioxidant effect on restraint stress induced altered free radical metabolism, rather a predominant effect similar to vitamin E alone was observed. The prevention of oxidative stress generated in response to restraint stress by the vitamins can be summarized as: vitamin (E + C) i.e. vit E > vit C > vit A, thus combined vitamin (E + C) treatment though showed maximum preventive effect, but was similar to vitamin E treatment alone, in terms of the circulating activities of SOD, GST, catalase and TBARS levels.

Original languageEnglish (US)
Pages (from-to)633-636
Number of pages4
JournalJournal of Nutritional Biochemistry
Volume14
Issue number11
DOIs
StatePublished - Jan 1 2003

Fingerprint

Vitamin E
Vitamins
Rats
Oxidative Stress
Antioxidants
Modulation
Ascorbic Acid
Thiobarbituric Acid Reactive Substances
Catalase
Vitamin A
Immobilization
Free Radicals
Metabolism
Rat control
Plasmas
Oxidative stress
Glutathione Transferase
Superoxide Dismutase
Body Weight

Keywords

  • Catalase
  • GST
  • Restraint stress
  • Superoxide dismutase
  • Vit A
  • Vit C
  • Vit E

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Modulation of restraint stress induced oxidative changes in rats by antioxidant vitamins. / Zaidi, S. M.Kashif R.; Al-Qirim, Tariq M.; Hoda, MD Nasrul; Banu, Naheed.

In: Journal of Nutritional Biochemistry, Vol. 14, No. 11, 01.01.2003, p. 633-636.

Research output: Contribution to journalArticle

Zaidi, S. M.Kashif R. ; Al-Qirim, Tariq M. ; Hoda, MD Nasrul ; Banu, Naheed. / Modulation of restraint stress induced oxidative changes in rats by antioxidant vitamins. In: Journal of Nutritional Biochemistry. 2003 ; Vol. 14, No. 11. pp. 633-636.
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