Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I

J. Lou, A. Ythier, D. Burger, L. Zheng, P. Juillard, Rudolf Lucas, J. M. Dayer, G. E. Grau

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

In this study, the effects of TNF binding protein I (TBP I) on TNF- induced changes of human brain microvascular endothelial cells (MVEC) were investigated. TBP I completely abolished TNF-induced IL-6 production and E- selectin induction, while it partially inhibited TNF-induced IL-8 production and up-regulation of ICAM-I and VCAM-I. Moreover, TBP I significantly inhibited TNF-induced cytotoxicity and leukocyte adherence on human brain MVEC. The inhibitory activity of TBP I for TNF was dose-dependent and related to the time of administration after TNF stimulation. In addition, TBP I inhibited membrane-bound TNF induced activation of human brain MVEC, but the concentration required was about 10-fold higher than that for soluble TNF. These results indicate a therapeutic potential for TBP I in diseases of the central nervous system associated with TNF overproduction.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalJournal of Neuroimmunology
Volume77
Issue number1
DOIs
StatePublished - Jul 1 1997
Externally publishedYes

Keywords

  • Adhesion molecules
  • Brain microvascular endothelial cells
  • Cytoadherence
  • Cytotoxicity
  • TNF
  • TNF binding protein I

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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