Molecular analysis of the gonadotropin-releasing hormone receptor in patients with polycystic ovary syndrome

Eytan M. Stein, Zhu Li, Christina K. Matulis, Lawrence C. Layman

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Objective: To determine whether a mutation in the GnRH receptor gene is responsible for polycystic ovary syndrome (PCOS). Design: Molecular analysis of human genomic DNA. Setting: Academic research environment. Patient(s): Eighty patients with PCOS. Intervention(s): Extraction and polymerase chain reaction (PCR) analysis of genomic DNA, confirmation of PCR products by ethidium bromide staining of agarose gels after electrophoresis, denaturing gradient gel electrophoresis of PCR products, and photography. Main Outcome Measure(s): Mutations in the GnRH receptor of women with PCOS. Result(s): Denaturing gradient gel electrophoresis revealed no mutations in the exonic sequence encoding the open reading frame of the GnRH receptor. Conclusion(s): A mutation in the GnRH receptor gene is unlikely to be the underlying cause of PCOS in most patients. The molecular basis of this disorder remains unknown.

Original languageEnglish (US)
Pages (from-to)360-363
Number of pages4
JournalFertility and sterility
Volume72
Issue number2
DOIs
StatePublished - Aug 1 1999

Keywords

  • GnRH
  • GnRH receptor
  • Polycystic ovary syndrome

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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