Molecular analysis of the gonadotropin-releasing hormone receptor in patients with polycystic ovary syndrome

Eytan M. Stein, Zhu Li, Christina K. Matulis, Lawrence C. Layman

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To determine whether a mutation in the GnRH receptor gene is responsible for polycystic ovary syndrome (PCOS). Design: Molecular analysis of human genomic DNA. Setting: Academic research environment. Patient(s): Eighty patients with PCOS. Intervention(s): Extraction and polymerase chain reaction (PCR) analysis of genomic DNA, confirmation of PCR products by ethidium bromide staining of agarose gels after electrophoresis, denaturing gradient gel electrophoresis of PCR products, and photography. Main Outcome Measure(s): Mutations in the GnRH receptor of women with PCOS. Result(s): Denaturing gradient gel electrophoresis revealed no mutations in the exonic sequence encoding the open reading frame of the GnRH receptor. Conclusion(s): A mutation in the GnRH receptor gene is unlikely to be the underlying cause of PCOS in most patients. The molecular basis of this disorder remains unknown.

Original languageEnglish (US)
Pages (from-to)360-363
Number of pages4
JournalFertility and sterility
Volume72
Issue number2
DOIs
StatePublished - Aug 1 1999

Fingerprint

LHRH Receptors
Polycystic Ovary Syndrome
Denaturing Gradient Gel Electrophoresis
Mutation
Polymerase Chain Reaction
Ethidium
Agar Gel Electrophoresis
Photography
DNA-Directed DNA Polymerase
Open Reading Frames
Genes
Outcome Assessment (Health Care)
Staining and Labeling
DNA
Research

Keywords

  • GnRH
  • GnRH receptor
  • Polycystic ovary syndrome

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Molecular analysis of the gonadotropin-releasing hormone receptor in patients with polycystic ovary syndrome. / Stein, Eytan M.; Li, Zhu; Matulis, Christina K.; Layman, Lawrence C.

In: Fertility and sterility, Vol. 72, No. 2, 01.08.1999, p. 360-363.

Research output: Contribution to journalArticle

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