Abstract
The transition from low grade astrocytoma to glioblastoma multiforme is almost always accompanied by the loss of genetic markers from chromosome 10. Recently two genes, PTEN/MMAC1/TEP1 and DMBT, have been isolated from chromosome 10q. We have analysed these two genes for mutations in 21 primary glioblastomas. An exon by exon screen of the PTEN gene using SSCP failed to identify any mutations in this tumour series. In contrast, 38% of tumours showed intragenic homozygous deletions in the DMBT gene. The fact that the majority of gliomas do not carry mutations in either of these genes suggests that there may still be other genes on chromosome 10 which are important in the development of glioblastoma multiforme.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1755-1757 |
| Number of pages | 3 |
| Journal | Oncogene |
| Volume | 17 |
| Issue number | 13 |
| DOIs | |
| State | Published - Oct 1 1998 |
| Externally published | Yes |
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Keywords
- 10q24-26
- DMBT
- Glioma
- Mutation
- PTEN
- Tumour suppressor
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research
Cite this
Molecular analysis of two putative tumour suppressor genes, PTEN and DMBT, which have been implicated in glioblastoma multiforme disease progression. / Somerville, R. P.T.; Shoshan, Y.; Eng, C.; Barnett, G.; Miller, D.; Cowell, J. K.
In: Oncogene, Vol. 17, No. 13, 01.10.1998, p. 1755-1757.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular analysis of two putative tumour suppressor genes, PTEN and DMBT, which have been implicated in glioblastoma multiforme disease progression
AU - Somerville, R. P.T.
AU - Shoshan, Y.
AU - Eng, C.
AU - Barnett, G.
AU - Miller, D.
AU - Cowell, J. K.
PY - 1998/10/1
Y1 - 1998/10/1
N2 - The transition from low grade astrocytoma to glioblastoma multiforme is almost always accompanied by the loss of genetic markers from chromosome 10. Recently two genes, PTEN/MMAC1/TEP1 and DMBT, have been isolated from chromosome 10q. We have analysed these two genes for mutations in 21 primary glioblastomas. An exon by exon screen of the PTEN gene using SSCP failed to identify any mutations in this tumour series. In contrast, 38% of tumours showed intragenic homozygous deletions in the DMBT gene. The fact that the majority of gliomas do not carry mutations in either of these genes suggests that there may still be other genes on chromosome 10 which are important in the development of glioblastoma multiforme.
AB - The transition from low grade astrocytoma to glioblastoma multiforme is almost always accompanied by the loss of genetic markers from chromosome 10. Recently two genes, PTEN/MMAC1/TEP1 and DMBT, have been isolated from chromosome 10q. We have analysed these two genes for mutations in 21 primary glioblastomas. An exon by exon screen of the PTEN gene using SSCP failed to identify any mutations in this tumour series. In contrast, 38% of tumours showed intragenic homozygous deletions in the DMBT gene. The fact that the majority of gliomas do not carry mutations in either of these genes suggests that there may still be other genes on chromosome 10 which are important in the development of glioblastoma multiforme.
KW - 10q24-26
KW - DMBT
KW - Glioma
KW - Mutation
KW - PTEN
KW - Tumour suppressor
UR - http://www.scopus.com/inward/record.url?scp=0032192306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032192306&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1202066
DO - 10.1038/sj.onc.1202066
M3 - Article
C2 - 9796706
AN - SCOPUS:0032192306
VL - 17
SP - 1755
EP - 1757
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 13
ER -