Molecular and functional characterization of the intestinal Na+- dependent multivitamin transporter

Puttur D Prasad, Haiping Wang, Wei Huang, You Jun Fei, Frederich H. Leibach, Lawrence D Devoe, Vadivel Ganapathy

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

We have cloned a Na+-dependent multivitamin transporter from rabbit intestine (riSMVT). The cDNA codes for a protein of 636 amino acids with 12 putative transmembrane domains. When expressed in mammalian cells, the cDNA induces Na+-dependent uptake of the vitamins pantothenate and biotin. Lipoate is also a substrate for the cDNA-induced uptake process. The affinity constant for the cDNA-specific transport of pantothenate and biotin is ~2 and ~8 μM, respectively. The Na+:vitamin stoichiometry is greater than 1, indicating that the transport process is electrogenic. The SMVT-specific transcripts of 3.2 kbp are equally distributed throughout the small intestine. We have also cloned SMVT from the human intestinal cell line Caco- 2. The Caco-2 SMVT cDNA codes for a protein of 635 amino acids which is homologous to riSMVT and is identical to the SMVT expressed in the human choriocarcinoma cell line JAR. Caco-2 SMVT also catalyzes Na+-dependent uptake of pantothenate, biotin, and lipoate. In oocytes expressing Caco-2 SMVT, all three vitamins evoke inward currents, confirming the electrogenicity of the transport process.

Original languageEnglish (US)
Pages (from-to)95-106
Number of pages12
JournalArchives of Biochemistry and Biophysics
Volume366
Issue number1
DOIs
StatePublished - Jun 1 1999

Fingerprint

Complementary DNA
Biotin
Vitamins
Cells
Amino Acids
Cell Line
Choriocarcinoma
Stoichiometry
Small Intestine
Oocytes
Intestines
Proteins
Rabbits
Substrates

Keywords

  • Biotin
  • Electrogenic transport
  • Intestine
  • Na/vitamin stoichiometry
  • Pantothenate
  • Primary structure
  • Vitamin transport

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Molecular and functional characterization of the intestinal Na+- dependent multivitamin transporter. / Prasad, Puttur D; Wang, Haiping; Huang, Wei; Fei, You Jun; Leibach, Frederich H.; Devoe, Lawrence D; Ganapathy, Vadivel.

In: Archives of Biochemistry and Biophysics, Vol. 366, No. 1, 01.06.1999, p. 95-106.

Research output: Contribution to journalArticle

Prasad, Puttur D ; Wang, Haiping ; Huang, Wei ; Fei, You Jun ; Leibach, Frederich H. ; Devoe, Lawrence D ; Ganapathy, Vadivel. / Molecular and functional characterization of the intestinal Na+- dependent multivitamin transporter. In: Archives of Biochemistry and Biophysics. 1999 ; Vol. 366, No. 1. pp. 95-106.
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