Molecular basis for hair loss in mice carrying a novel nonsense mutation (Hrrh-R) in the hairless gene (Hr)

Yutao Liu, J. P. Sundberg, S. Das, D. Carpenter, K. T. Cain, E. J. Michaud, B. H. Voy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Animal models carrying mutations in the hairless (Hr) gene provide a rich resource for study of hair follicle biology. A spontaneous mouse mutant with a phenotype strikingly similar to rhino mutants of Hr arose spontaneously in the mouse facility at Oak Ridge National Laboratory. Sequence analysis of Hr in these mutants uncovered a nonsense mutation in exon 12, designated as Hrrh-R (rhino, Oak Ridge). The mutation led to significant reduction in Hr mRNA levels, predicted to be due to nonsense-mediated decay. Histological analysis indicated dilated hair follicle infundibula at 14 days of age that rapidly became filled with cornified material. Microarray analyses revealed that expression levels of many genes involved in keratinocyte differentiation, epidermal regeneration, and wound healing were significantly upregulated before morphological detection of the phenotype, suggesting their role in onset of the Hrrh-R phenotype. Identification of this new Hr allele and the underlying molecular alterations allows further understanding of the role of Hr in hair follicle biology.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalVeterinary Pathology
Volume47
Issue number1
DOIs
StatePublished - Jun 25 2010
Externally publishedYes

Fingerprint

nonsense mutation
hair follicles
Hair Follicle
alopecia
Nonsense Codon
Alopecia
Phenotype
phenotype
mutants
mice
Quercus
Genes
mutation
Biological Sciences
Mutation
genes
keratinocytes
Pituitary Gland
Microarray Analysis
tissue repair

Keywords

  • Alopecia
  • Hair follicle
  • Hairless mice
  • Microarray analysis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular basis for hair loss in mice carrying a novel nonsense mutation (Hrrh-R) in the hairless gene (Hr). / Liu, Yutao; Sundberg, J. P.; Das, S.; Carpenter, D.; Cain, K. T.; Michaud, E. J.; Voy, B. H.

In: Veterinary Pathology, Vol. 47, No. 1, 25.06.2010, p. 167-176.

Research output: Contribution to journalArticle

Liu, Yutao ; Sundberg, J. P. ; Das, S. ; Carpenter, D. ; Cain, K. T. ; Michaud, E. J. ; Voy, B. H. / Molecular basis for hair loss in mice carrying a novel nonsense mutation (Hrrh-R) in the hairless gene (Hr). In: Veterinary Pathology. 2010 ; Vol. 47, No. 1. pp. 167-176.
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