TY - JOUR
T1 - Molecular basis for heterosis for myelin basic protein content in mice
AU - Miskimins, R.
AU - Ebato, H.
AU - Seyfried, T. N.
AU - Yu, R. K.
PY - 1986
Y1 - 1986
N2 - Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 x D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the same efficiency with respect to stimulation of amino acid incorporation into protein. However, a significant heterotic effect was seen for the production of myelin basic protein (MBP) by the mRNA from the F1 mice. That is, the fraction of protein synthesized as MBP was greater for the F1 hybrid than for either parental strain. The distribution of the form of MBPs was not different among the three strains. We therefore believe that heterosis for brain MBP content in the F1 hybrid may be regulated at the transcriptional or post-transcriptional level.
AB - Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 x D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the same efficiency with respect to stimulation of amino acid incorporation into protein. However, a significant heterotic effect was seen for the production of myelin basic protein (MBP) by the mRNA from the F1 mice. That is, the fraction of protein synthesized as MBP was greater for the F1 hybrid than for either parental strain. The distribution of the form of MBPs was not different among the three strains. We therefore believe that heterosis for brain MBP content in the F1 hybrid may be regulated at the transcriptional or post-transcriptional level.
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U2 - 10.1073/pnas.83.5.1532
DO - 10.1073/pnas.83.5.1532
M3 - Article
C2 - 2419910
AN - SCOPUS:0022523263
SN - 0027-8424
VL - 83
SP - 1532
EP - 1535
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 5
ER -