Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis

David J Kozlowski, Zhong Chen, Lina Zhuang, You Jun Fei, Sammy Navarre, Vadivel Ganapathy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na+/Cl--dependent manner with a Na+:Cl-:taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates.

Original languageEnglish (US)
Pages (from-to)1004-1011
Number of pages8
JournalLife Sciences
Volume82
Issue number19-20
DOIs
StatePublished - May 7 2008

Fingerprint

Zebrafish
Embryonic Development
Taurine
taurine transporter
Morpholinos
Messenger RNA
Antisense Oligonucleotides
Blood vessels
Neurology
Blastocyst
Cell death
Stoichiometry
Blood Vessels
Vertebrates
Retina
Brain
Cell Death
Embryonic Structures
Central Nervous System
Complementary DNA

Keywords

  • Apoptosis
  • Brain
  • Embryogenesis
  • Retina
  • Taurine transporter
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis. / Kozlowski, David J; Chen, Zhong; Zhuang, Lina; Fei, You Jun; Navarre, Sammy; Ganapathy, Vadivel.

In: Life Sciences, Vol. 82, No. 19-20, 07.05.2008, p. 1004-1011.

Research output: Contribution to journalArticle

Kozlowski, David J ; Chen, Zhong ; Zhuang, Lina ; Fei, You Jun ; Navarre, Sammy ; Ganapathy, Vadivel. / Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis. In: Life Sciences. 2008 ; Vol. 82, No. 19-20. pp. 1004-1011.
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