Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis

David J Kozlowski; Zhong Chen; Lina Zhuang; You Jun Fei; Sammy Navarre; Vadivel Ganapathy

doi:10.1016/j.lfs.2008.02.015

Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis

David J Kozlowski, Zhong Chen, Lina Zhuang, You Jun Fei, Sammy Navarre, Vadivel Ganapathy

Neuroscience and Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na⁺/Cl^--dependent manner with a Na⁺:Cl^-:taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates.

Original language	English (US)
Pages (from-to)	1004-1011
Number of pages	8
Journal	Life sciences
Volume	82
Issue number	19-20
DOIs	https://doi.org/10.1016/j.lfs.2008.02.015
State	Published - May 7 2008

Keywords

Apoptosis
Brain
Embryogenesis
Retina
Taurine transporter
Zebrafish

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.lfs.2008.02.015

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title = "Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis",

abstract = "Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na+/Cl--dependent manner with a Na+:Cl-:taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates.",

keywords = "Apoptosis, Brain, Embryogenesis, Retina, Taurine transporter, Zebrafish",

author = "Kozlowski, {David J} and Zhong Chen and Lina Zhuang and Fei, {You Jun} and Sammy Navarre and Vadivel Ganapathy",

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AU - Kozlowski, David J

AU - Chen, Zhong

AU - Zhuang, Lina

AU - Fei, You Jun

AU - Navarre, Sammy

AU - Ganapathy, Vadivel

PY - 2008/5/7

Y1 - 2008/5/7

N2 - Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na+/Cl--dependent manner with a Na+:Cl-:taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates.

AB - Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na+/Cl--dependent manner with a Na+:Cl-:taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates.

KW - Apoptosis

KW - Brain

KW - Embryogenesis

KW - Retina

KW - Taurine transporter

KW - Zebrafish

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Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis

Abstract

Keywords

ASJC Scopus subject areas

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