Molecular characterization of fibroblasts isolated from human peritoneum and adhesions

Ghassan M. Saed, Wendy Zhang, Michael Peter Diamond

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Objective: To determine the response of adhesion and peritoneal fibroblasts to hypoxia. Design: Prospective experimental study. Setting: University medical center. Patient(s): Primary cultures of fibroblasts established from the peritoneal and adhesion tissues of the same patients (n = 2) to minimize genetic variations.Intervention(s): Hypoxia treatment of the primary cultured fibroblast. Main Outcome Measure(s): Analyze the expression of extracellular matrix (ECM) components, metalloproteinases and their tissue inhibitors, growth factors, and cytokines in adhesion and peritoneal fibroblasts under normal and hypoxic conditions by reverse transcriptase/polymerase chain reaction analysis. Result(s): Compared to peritoneal fibroblasts, adhesion fibroblasts had a significant increase in the basal mRNA levels for collagen I, fibronectin, MMP-1, TIMP-1, TGF-β1, TGF-β2, and IL-10. Hypoxia resulted in a further increase in collagen 1, fibronectin, TIMP-1, TGF-β1, TGF-β2, IL-10, and IFN-γ mRNA levels in both peritoneal and adhesion fibroblasts. The increase was more profound in adhesion fibroblasts. Conclusion(s): Hypoxia induces molecular changes in both peritoneal and adhesion fibroblasts, creating a milieu that favors adhesion development. The effect of hypoxia was more profound on adhesion fibroblasts.

Original languageEnglish (US)
Pages (from-to)763-768
Number of pages6
JournalFertility and Sterility
Volume75
Issue number4
DOIs
StatePublished - Apr 18 2001
Externally publishedYes

Fingerprint

Peritoneum
Fibroblasts
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinases
Fibronectins
Interleukin-10
Collagen
Tissue Adhesions
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Research Design
Outcome Assessment (Health Care)
Hypoxia
Prospective Studies
Cytokines

Keywords

  • Adhesion
  • Fibroblasts
  • Hypoxia
  • Peritoneum
  • RT/PCR

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Molecular characterization of fibroblasts isolated from human peritoneum and adhesions. / Saed, Ghassan M.; Zhang, Wendy; Diamond, Michael Peter.

In: Fertility and Sterility, Vol. 75, No. 4, 18.04.2001, p. 763-768.

Research output: Contribution to journalArticle

@article{fc7a24c0a80a469cae36c3054043c907,
title = "Molecular characterization of fibroblasts isolated from human peritoneum and adhesions",
abstract = "Objective: To determine the response of adhesion and peritoneal fibroblasts to hypoxia. Design: Prospective experimental study. Setting: University medical center. Patient(s): Primary cultures of fibroblasts established from the peritoneal and adhesion tissues of the same patients (n = 2) to minimize genetic variations.Intervention(s): Hypoxia treatment of the primary cultured fibroblast. Main Outcome Measure(s): Analyze the expression of extracellular matrix (ECM) components, metalloproteinases and their tissue inhibitors, growth factors, and cytokines in adhesion and peritoneal fibroblasts under normal and hypoxic conditions by reverse transcriptase/polymerase chain reaction analysis. Result(s): Compared to peritoneal fibroblasts, adhesion fibroblasts had a significant increase in the basal mRNA levels for collagen I, fibronectin, MMP-1, TIMP-1, TGF-β1, TGF-β2, and IL-10. Hypoxia resulted in a further increase in collagen 1, fibronectin, TIMP-1, TGF-β1, TGF-β2, IL-10, and IFN-γ mRNA levels in both peritoneal and adhesion fibroblasts. The increase was more profound in adhesion fibroblasts. Conclusion(s): Hypoxia induces molecular changes in both peritoneal and adhesion fibroblasts, creating a milieu that favors adhesion development. The effect of hypoxia was more profound on adhesion fibroblasts.",
keywords = "Adhesion, Fibroblasts, Hypoxia, Peritoneum, RT/PCR",
author = "Saed, {Ghassan M.} and Wendy Zhang and Diamond, {Michael Peter}",
year = "2001",
month = "4",
day = "18",
doi = "10.1016/S0015-0282(00)01799-4",
language = "English (US)",
volume = "75",
pages = "763--768",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Molecular characterization of fibroblasts isolated from human peritoneum and adhesions

AU - Saed, Ghassan M.

AU - Zhang, Wendy

AU - Diamond, Michael Peter

PY - 2001/4/18

Y1 - 2001/4/18

N2 - Objective: To determine the response of adhesion and peritoneal fibroblasts to hypoxia. Design: Prospective experimental study. Setting: University medical center. Patient(s): Primary cultures of fibroblasts established from the peritoneal and adhesion tissues of the same patients (n = 2) to minimize genetic variations.Intervention(s): Hypoxia treatment of the primary cultured fibroblast. Main Outcome Measure(s): Analyze the expression of extracellular matrix (ECM) components, metalloproteinases and their tissue inhibitors, growth factors, and cytokines in adhesion and peritoneal fibroblasts under normal and hypoxic conditions by reverse transcriptase/polymerase chain reaction analysis. Result(s): Compared to peritoneal fibroblasts, adhesion fibroblasts had a significant increase in the basal mRNA levels for collagen I, fibronectin, MMP-1, TIMP-1, TGF-β1, TGF-β2, and IL-10. Hypoxia resulted in a further increase in collagen 1, fibronectin, TIMP-1, TGF-β1, TGF-β2, IL-10, and IFN-γ mRNA levels in both peritoneal and adhesion fibroblasts. The increase was more profound in adhesion fibroblasts. Conclusion(s): Hypoxia induces molecular changes in both peritoneal and adhesion fibroblasts, creating a milieu that favors adhesion development. The effect of hypoxia was more profound on adhesion fibroblasts.

AB - Objective: To determine the response of adhesion and peritoneal fibroblasts to hypoxia. Design: Prospective experimental study. Setting: University medical center. Patient(s): Primary cultures of fibroblasts established from the peritoneal and adhesion tissues of the same patients (n = 2) to minimize genetic variations.Intervention(s): Hypoxia treatment of the primary cultured fibroblast. Main Outcome Measure(s): Analyze the expression of extracellular matrix (ECM) components, metalloproteinases and their tissue inhibitors, growth factors, and cytokines in adhesion and peritoneal fibroblasts under normal and hypoxic conditions by reverse transcriptase/polymerase chain reaction analysis. Result(s): Compared to peritoneal fibroblasts, adhesion fibroblasts had a significant increase in the basal mRNA levels for collagen I, fibronectin, MMP-1, TIMP-1, TGF-β1, TGF-β2, and IL-10. Hypoxia resulted in a further increase in collagen 1, fibronectin, TIMP-1, TGF-β1, TGF-β2, IL-10, and IFN-γ mRNA levels in both peritoneal and adhesion fibroblasts. The increase was more profound in adhesion fibroblasts. Conclusion(s): Hypoxia induces molecular changes in both peritoneal and adhesion fibroblasts, creating a milieu that favors adhesion development. The effect of hypoxia was more profound on adhesion fibroblasts.

KW - Adhesion

KW - Fibroblasts

KW - Hypoxia

KW - Peritoneum

KW - RT/PCR

UR - http://www.scopus.com/inward/record.url?scp=0035064828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035064828&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(00)01799-4

DO - 10.1016/S0015-0282(00)01799-4

M3 - Article

VL - 75

SP - 763

EP - 768

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 4

ER -