Molecular characterization of fibroblasts isolated from human peritoneum and adhesions

Ghassan M. Saed, Wendy Zhang, Michael P. Diamond

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

Objective: To determine the response of adhesion and peritoneal fibroblasts to hypoxia. Design: Prospective experimental study. Setting: University medical center. Patient(s): Primary cultures of fibroblasts established from the peritoneal and adhesion tissues of the same patients (n = 2) to minimize genetic variations.Intervention(s): Hypoxia treatment of the primary cultured fibroblast. Main Outcome Measure(s): Analyze the expression of extracellular matrix (ECM) components, metalloproteinases and their tissue inhibitors, growth factors, and cytokines in adhesion and peritoneal fibroblasts under normal and hypoxic conditions by reverse transcriptase/polymerase chain reaction analysis. Result(s): Compared to peritoneal fibroblasts, adhesion fibroblasts had a significant increase in the basal mRNA levels for collagen I, fibronectin, MMP-1, TIMP-1, TGF-β1, TGF-β2, and IL-10. Hypoxia resulted in a further increase in collagen 1, fibronectin, TIMP-1, TGF-β1, TGF-β2, IL-10, and IFN-γ mRNA levels in both peritoneal and adhesion fibroblasts. The increase was more profound in adhesion fibroblasts. Conclusion(s): Hypoxia induces molecular changes in both peritoneal and adhesion fibroblasts, creating a milieu that favors adhesion development. The effect of hypoxia was more profound on adhesion fibroblasts.

Original languageEnglish (US)
Pages (from-to)763-768
Number of pages6
JournalFertility and sterility
Volume75
Issue number4
DOIs
StatePublished - Apr 18 2001
Externally publishedYes

Keywords

  • Adhesion
  • Fibroblasts
  • Hypoxia
  • Peritoneum
  • RT/PCR

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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